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Increasing evidence suggests that heat acclimation and exogenous salicylic acid (SA) and abscisic acid (ABA) may lead to the enhancement of thermotolerance in plants. In this study, the roles that free SA, conjugated SA, ABA, and phosphatidylinositol-4,5-bisphosphate (PIP(2))-specific phospholipase C (PLC) play in thermotolerance development induced by heat(More)
Stilbene synthase (STS, EC leads to the production of resveratrol compounds, which are major components of the phytoalexin response against fungal pathogens of the plant and are highly bioactive substances of pharmaceutical interest. STS expression and regulation are important. Temperature is one of the main external factors affecting phytoalexin(More)
The relationship between the accumulation in endogenous free salicylic acid (SA) induced by heat acclimation (37 degrees C) and the activity of PIP(2)-phospholipase C (PIP(2)-PLC; EC in the plasma membrane fraction was investigated in pea (Pisum sativum L.) leaves. We focused our attention on the hypothesis that positive SA signals induced by heat(More)
Both phospholipase D (PLD, EC and salicylic acid (SA) play important roles in response to external stimulation and activating defense system in plants. However, roles of the two signals in plants during the development of thermotolerance induced by low temperature acclimation remain unclear. In the experiment presented in the paper, grape berries(More)
Cell suspension cultures of Vitis vinifera were treated with chitosan and ultraviolet C (UV-C) irradiation, alone or in combination, to investigate the relationship between the production of stilbene and the transcript profiles of genes encoding the enzymes involved in stilbene synthesis. Treatment at the proper concentration or dosage enhanced both(More)
To explore the effects of harvest time on phenolic compounds during wine fermentation, grape berries (Vitis vinifera L. cv. Vidal) were harvested at 17.5, 22.8 and 37.2 masculine Brix and were used to make dry wine, semi-sweet wine and icewine with low alcohol levels, respectively. Phenolic acids and flavan-3-ols were assayed during the fermentation of(More)
Plants have evolved mechanisms to avoid and repair UV radiation damage, and the free radicals caused by UV tend to be involved in the induction of antioxidant defense systems. In this study, changes in resveratrol and antioxidant enzymes were investigated in relation to UV damage in peanut seedlings. Accumulation of endogenous resveratrol and stilbene(More)
Vitis vinifera L. cv. Cabernet Sauvignon cell suspension cultures were treated with ultraviolet C (UV-C), methyl jasmonate (MeJA) and salicylic acid (SA), alone or in combination, to investigate the effects on stilbene biosynthesis. The application of elicitors at the proper dosage or concentration did not exert a negative effect on cell growth. All(More)
To investigate whether phospholipase D (PLD, EC plays a role in adaptive response of post-harvest fruit to environment, a PLD gene was firstly cloned from grape berry (Vitis Vinifera L. cv. Chardonnay) using RT-PCR and 3'- and 5'-RACE. The deduced amino acid sequence (809 residues) showed 84.7% identity with that of PLD from Ricinus communis. The(More)
The subcellular distribution and activity of glucose-6-phosphate dehydrogenase (G6PDH, EC were studied in developing peach (Prunus persica L. Batsch cv. Zaoyu) fruit. Fruit tissues were separated by differential centrifugation at 15,000g into plastidic and cytosolic fractions. There was no serious loss of enzyme activity (or activation) during the(More)