Ji Sheng Nie

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We present a highly sensitive and selective chemical labeling and capture approach for genome-wide profiling of 5-hydroxylmethylcytosine (5hmC) using DNA isolated from ∼1,000 cells (nano-hmC-Seal). Using this technology, we assessed 5hmC occupancy and dynamics across different stages of hematopoietic differentiation. Nano-hmC-Seal profiling of purified(More)
7 Responses of shallow cumuli to large-scale temperature/moisture perturbations are examined 8 through diagnostics of large-eddy-simulations (LES) of the undisturbed Barbados Oceano9 graphic and Meteorological Experiment (BOMEX) case and a stochastic parcel model. The 10 perturbations are added instantaneously and allowed to evolve freely afterwards. The(More)
Enzyme linked aptamer assay (ELAA) uses an aptamer as recognition element and enzyme as signal readout element for establishing different kinds of aptasensors. We reported herein a high-throughput colorimetric aptasensor based on ELAA only requiring a single aptamer sequence for cocaine detection. An anti-cocaine aptamer was cleaved into two fragments, one(More)
[1] A new framework for diagnosing mixing in cumulus convection is proposed, where the vertical evolution of the cloud mass flux distribution as a function of given parcel properties is viewed as a mapping process. Matrices that characterize this mapping process are constructed using a Lagrangian particle dispersion model embedded in a Large Eddy(More)
Idealized dynamical theories that employ a convective quasi-equilibrium (QE) treatment for the diabatic effects of moist convection have been used to explain the location, intensity, and intraseasonal evolution of monsoons. This paper examines whether observations of Earth’s regional monsoons are consistent with the assumption of QE. It is shown here that(More)
A simple and novel reporter-triggered isothermal exponential amplification reaction (R-EXPAR) integrated with a miniaturized electrochemical device was developed, which achieved excellent improvement (five orders of magnitude) of sensitivity toward reporter, G-quadruplex. This R-EXPAR strategy has been successfully implemented to construct a homogeneous(More)
Hyperhomocysteinemia, which is characterized by elevated blood levels of the non-protein amino acid homocysteine (Hcy), is an independent risk factor for many diseases, including cardiovascular diseases, neurodegenerative diseases and birth defects. The incorporation of homocysteine into proteins, known as protein N-homocysteinylation, has been considered a(More)
Herein, G-quadruplex sequence was found to significantly decrease the diffusion current of methylene blue (MB) in homogeneous solution for the first time. Electrochemical methods combined with circular dichroism spectroscopy and UV-vis spectroscopy were utilized to systematically explore the interaction between MB and an artificial G-quadruplex sequence,(More)
4 A single-column modeling approach is proposed to study interaction between convection and 5 large-scale dynamics using the quasi-geostrophic (QG) framework. Vertical motion is repre6 sented by the QG omega equation with the diabatic heating term included. This approach 7 extends the notion of “parameterization of large scale dynamics”, previously applied(More)
A newly developed sheathless interface for capillary electrophoresis-mass spectrometry, using a porous tip sprayer, was first applied for highly sensitive determination of cytosine modifications. The system performed well in identification and quantification of both 5-methylcytosine and 5-hydroxymethylcytosine using only 125 pg (∼20 cells) genomic DNA(More)