Jiřina Dvořáková

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Kinetics of phytate hydrolysis byAspergillus niger phytase and correlation between the amount of released phosphate and creation of lowermyo-inositol phosphates were investigated. Phytase was able to hydrolyzemyo-inositol hexakis-, pentakis-, tetrakis-, and trisphosphates. Finally, about 56% of total phosphate were released andmyo-inositol bisphosphate was(More)
Levels of most of the examined proteins in cerebrospinal fluid (CSF) of 107 patients with neuroborreliosis were associated with cytological findings, the status of hematoencephalic barrier as evaluated byQ alb (cerebrospinal fluid to serum quotient) and the intrathecal synthesis of immunoglobulins. Cytological findings consisted of normal cytology, or both(More)
The extracellular activity ofAspergillus niger phytase at the end of the growth phase was 132 nkat/mL in a laboratory bioreactor. The purified enzyme has molar mass approximately 100 kDa, pH optimum at 5.0, temperature optimum at 55°C and high pH and temperature stability. TheK m for dodecasodium phytate, calcium phytate and 4-nitrophenyl phosphate are(More)
Aerobically grown cells ofParacoccus denitrificans exhibited a marked increase in the activity of 5-aminolevulinate synthase in the early stage of adaptation to the decreased aeration of the growth medium. The effect was dependent on the functional proteosynthesis; it was not influenced by the presence of nitrate in the medium. An enhancement of nitrate(More)
Electrophoretic analysis of alcohol oxidase purified from the methylotrophic thermo- and acidotolerant yeastHansenula sp. revealed the presence of two active forms of the enzyme with molar mass 440 kg/mol (major component) and 724 kg/mol (minor component). A subunitM of the enzyme was found to be 72 kg/mol. Two active forms of the enzyme found by(More)
The effect of repeated Azosemide infusions (20 mg in 500 ml 5% glucose for one h) on urine volume and electrolyte excretion, and on the activity of the renin-angiotensin-aldosterone system (RAAS) was studied in a group of 15 patients with benign essential hypertension before and during treatment with the beta-adrenergic blocker Trimepranol. Azosemide alone(More)
The urinary excretion of free noradrenaline (NA), adrenaline (A), dopamine (DA), the DA/NA ratio in the urine, plasma renin activity (PRA) and their mutual relationship were investigated in 71 patients suffering from different types of arterial hypertension. In spite of the fact that the mean values of excreted catecholamines, with the exception of(More)
A single dose of clomipramine, 10 mg i.v., or desipramine, 25 mg i.m., was administered to seven healthy young sibling pairs in a randomized cross-over experiment. The response of serum growth hormone, prolactin and cortisol was measured. The main findings were (1) sex differences in the growth hormone and cortisol response to desipramine and (2) a(More)