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Holistic analysis of lipids is becoming increasingly popular in the life sciences. Recently, several interesting, mass spectrometry-based studies have been conducted, especially in plant biology. However, while great advancements have been made we are still far from detecting all the lipids species in an organism. In this study we developed an ultra(More)
We applied a top-down systems biology approach to understand how Chlamydomonas reinhardtii acclimates to long-term heat stress (HS) and recovers from it. For this, we shifted cells from 25 to 42°C for 24 h and back to 25°C for ≥8 h and monitored abundances of 1856 proteins/protein groups, 99 polar and 185 lipophilic metabolites, and cytological and(More)
The paper presents the ultra-performance liquid chromatography (UPLC) and high-resolution mass spectrometric analysis and comparison of total lipid profiles of two green algal species, Chlamydomonas reinhardtii and Scenedesmus (Acutodesmus) obliquus. The targeted UPLC-mass spectroscopy (MS) analysis revealed that both the green algae showed the presence of(More)
The elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emerged to provide the tools for successful system-wide investigations. Unfortunately, optimized analysis of different compounds requires specific(More)
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