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Engineering Cytochrome P450 Enzymes for Improved Activity towards Biomimetic 1,4‐NADH Cofactors
Cytochrome P450s are heme-containing enzymes that can oxidize a broad range of compounds, often at unreactive carbon hydrogen bonds, with high regioand enantioselectivity. Two bacterial P450s, theExpand
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High-Throughput and Combinatorial Gene Expression on a Chip for Metabolism-Induced Toxicology Screening
Differential expression of various drug-metabolizing enzymes in the human liver may cause deviations of pharmacokinetic profiles, resulting in inter-individual variability of drug toxicity and/orExpand
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  • Open Access
Rapid and quantitative measurement of metabolic stability without chromatography or mass spectrometry.
Metabolic stability measurements are a critical component of preclinical drug development. Available measurement strategies rely on chromatography and mass spectrometry, which are expensive and laborExpand
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Esterase activity of bovine serum albumin up to 160 °C: A new benchmark for biocatalysis
Abstract Albumins are highly conserved proteins that carry out a multitude of physiological functions and exhibit a broad range of catalytic activities. Moreover, amino acid sequence comparisons ofExpand
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Cell-based screen of HMG-CoA reductase inhibitors and expression regulators using LC-MS.
We present an integrated consolidation of previously reported methods for screening hydroxymethylglutaryl-coenzyme A reductase (HMGR) inhibitors in 96-well microtiter plates with rapid workup usingExpand
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P450cam biocatalysis in surfactant‐stabilized two‐phase emulsions
Cytochrome P450 monooxygenases (P450s) are powerful biocatalysts that have the ability to oxidize a broad range of substrates, often at non‐reactive carbon centers. However, incorporation of P450sExpand
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Bioorganometallic chemistry: Co-factor regeneration, enzyme recognition of biomimetic 1,4-NADH analogs, and organic synthesis; tandem catalyzed regioselective formation of
Abstract Two novel tandem catalysis approaches for the chiral synthesis of S-alcohols from reduction of their prochiral ketones with Horse Liver Alcohol Dehydrogenase (HLADH), and selective C-HExpand
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Microarray biochips for high-throughput drug screening