• Publications
  • Influence
Targeted Quantitation of Site-Specific Cysteine Oxidation in Endogenous Proteins Using a Differential Alkylation and Multiple Reaction Monitoring Mass Spectrometry Approach
TLDR
A highly sensitive and reproducible oxidation analysis approach that combines protein purification, differential alkylation with stable isotopes, and multiple reaction monitoring mass spectrometry that can be applied in a targeted manner to virtually any cysteine or protein is described. Expand
Glutathionylation of the Active Site Cysteines of Peroxiredoxin 2 and Recycling by Glutaredoxin*
TLDR
It is concluded that Prx2 glutathionylation is a favorable reaction that can occur in cells under oxidative stress and may have a role in redox signaling. Expand
Overexpression of Catalase Diminishes Oxidative Cysteine Modifications of Cardiac Proteins
TLDR
The data suggest that catalase may alleviate cardiac disease and aging by moderating global protein cysteine thiol oxidation. Expand
Upregulation of Glutaredoxin-1 Activates Microglia and Promotes Neurodegeneration: Implications for Parkinson's Disease.
TLDR
In vitro and in vivo data suggest Grx1 upregulation promotes neurotoxic neuroinflammation, potentially contributing to PD, and synergizes with proinflammatory insults to promote DA loss in vivo. Expand
Mitochondrial Reactive Oxygen Species Mediate Cardiac Structural, Functional, and Mitochondrial Consequences of Diet‐Induced Metabolic Heart Disease
TLDR
Mitochondrial ROS are pathogenic in MHD and contribute to mitochondrial dysfunction, at least in part, by causing oxidative posttranslational modifications of complex I and II proteins including reversible oxidative post translational modification of complex II subunit B Cys100 and Cys103. Expand
High fat, high sucrose diet causes cardiac mitochondrial dysfunction due in part to oxidative post-translational modification of mitochondrial complex II.
TLDR
MHD due to consumption of a HFHS "Western" diet causes increased H2O2 production and oxidative stress in cardiac mitochondria associated with decreased ATP synthesis and decreased complex II activity. Expand
Does reversible cysteine oxidation link the Western diet to cardiac dysfunction?
TLDR
The findings suggest that the metabolic syndrome leads to potentially deleterious changes in the oxidative modification of metabolically active proteins, which may adversely regulate energy substrate flux through glycolysis, β‐oxidation, citric acid (TCA) cycle, and oxidative phosphorylation (oxphos), thereby contributing to maladaptive tissue remodeling that is associated with, and possibly contributing to, diastolic left ventricular dysfunction. Expand
Spatial and temporal alterations in protein structure by EGF regulate cryptic cysteine oxidation
TLDR
The novel finding that cryptic cysteines are redox regulated has important implications for how redox signaling networks are specified and regulated to minimize crosstalk. Expand
Glutaredoxin-1 Deficiency Causes Fatty Liver and Dyslipidemia by Inhibiting Sirtuin-1.
TLDR
An essential role of hepatic Glrx in regulating SirT1, which controls protein glutathione adducts in the pathogenesis of hepatics steatosis is suggested, and it is proposed that upregulation of liver-based Glrx may be a beneficial strategy for NAFL. Expand
Label-Free Quantitation and Mapping of the ErbB2 Tumor Receptor by Multiple Protease Digestion with Data-Dependent (MS1) and Data-Independent (MS2) Acquisitions
TLDR
Combining high resolution proteomics with multiprotease digestion enabled quantitative mapping of ErbB2 with excellent reproducibility, improved amino acid sequence and PTM coverage, and decreased assay development time compared to typical SRM assays, demonstrating that high resolution quantitative proteomic approaches are an effective tool for targeted biomarker quantitation. Expand
...
1
2
...