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SUMMARY Adenosine diphosphate was found to be a powerful competitive inhibitor of 5'nucleotidase partially purified from rat heart, with a greater apparent affinity for the enzyme than ATP. The inhibition imposed by either nucleotide was not relieved by a number of metabolic intermediates known to accumulate in the hypoxic heart. The enzyme required(More)
The cytosolic pools of glucose-1-phosphate (Glc-1-P) and glucose-6-phosphate are essential intermediates in several biosynthetic paths, including the formation of sucrose and cell wall constituents, and they are also linked to the cytosolic starch-related heteroglycans. In this work, structural features and biochemical properties of starch-related(More)
This book chapter describes the analytical procedures required for the profiling of a metabolite fraction enriched for primary metabolites. The profiling is based on routine gas chromatography coupled to mass spectrometry (GC-MS). The generic profiling method is adapted to plant material, specifically to the analysis of single leaves from plants that were(More)
Phosphofructokinase (EC 2.7.1.11) is subject to inactivation (at pH values below 6) and reactivation (at alkaline pH) to forms that correspond, respectively, to immature and mature conformational states. ATP is one of several ligands that facilitate the reactivation process, which we have termed preconditioning (1, 2). Evidence is presented that the role of(More)