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C2 domains are widespread Ca2+-binding modules. The active zone protein Piccolo (also known as Aczonin) contains an unusual C2A domain that exhibits a low affinity for Ca2+, a Ca2+-induced conformational change and Ca2+-dependent dimerization. We show here that removal of a nine-residue sequence by alternative splicing increases the Ca2+ affinity, abolishes(More)
Synaptotagmin 1, a Ca2+ sensor for fast synaptic vesicle exocytosis, contains two C2 domains that form Ca2+-dependent complexes with phospholipids. To examine the functional importance of Ca2+ binding to the C2A domain of synaptotagmin 1, we studied two C2A domain mutations, D232N and D238N, using recombinant proteins and knock-in mice. Both mutations(More)
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The recent interest in the surveillance of public, military, and commercial scenarios is increasing the need to develop and deploy intelligent and/or automated distributed visual surveillance systems. Many applications based on distributed resources use the so-called software agent technology. In this paper, a multi-agent framework is applied to coordinate(More)
Voltage-sensitive calcium channels (VSCCs) mediate signaling events in bone cells in response to mechanical loading. Osteoblasts predominantly express L-type VSCCs composed of the α(1) pore-forming subunit and several auxiliary subunits. Osteocytes, in contrast, express T-type VSCCs and a relatively small amount of L-type α(1) subunits. Auxiliary VSCC(More)
The off rate from the first calcium-binding site of the C2A domain of synaptotagmin I, a putative calcium receptor in neurotransmitter release, has been determined by 15N-nuclear magnetic resonance relaxation dispersion measurements. The exchange rate was obtained by fitting the dependence of the transverse relaxation rates on the interval between 180(More)
This paper presents an application of software agents in a specific engineering problem: multitarget surveillance with several cameras sensing a closed environment. Each agent controls a camera , and from the coordination of them will emerge an improvement of the global surveillance task. Agents emulate human camera operators and, therefore human-like(More)
Maritime surveillance involves gathering and integrating a large amount of heterogeneous information of variable quality to provide diverse decision makers with reliable knowledge about situations and threats. This requires information processing at all fusion levels while taking into account contextual information. Context is especially important for(More)
Contextual Information is proving to be not only an additional exploitable information source for improving entity and situational estimates in certain Information Fusion systems, but can also be the entire focus of estimation for such systems as those directed to Ambient Intelligence (AI) and Context-Aware(CA) applications. This paper will discuss the(More)