Jeryl L. Appleby

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four-step His-Asp-His-Asp phosphorelay. Burbulys et Signal Transduction by Two-Component al. (1991) reported the first known phosphorelay built Regulatory Systems from sensor kinase and response regulator family memAll living cells must sense changes in their environment bers. This pathway, which governs initiation of sporulaand respond appropriately. To(More)
four-step His-Asp-His-Asp phosphorelay. Burbulys et Signal Transduction by Two-Component al. (1991) reported the first known phosphorelay built Regulatory Systems from sensor kinase and response regulator family memAll living cells must sense changes in their environment bers. This pathway, which governs initiation of sporulaand respond appropriately. To(More)
CheY serves as a structural prototype for the response regulator proteins of two-component regulatory systems. Functional roles have previously been defined for four of the five highly conserved residues that form the response regulator active site, the exception being the hydroxy amino acid which corresponds to Thr87 in CheY. To investigate the(More)
The site of phosphorylation of the chemotaxis response regulator CheY is aspartate 57. When Asp-57 is replaced with an asparagine, the resultant protein can be phosphorylated at an alternative site. We report here that phosphorylation of this mutant protein, CheY D57N, at the alternative site affords the protein activity in vivo in the absence of CheZ.(More)
Kinetic and equilibrium measurements of phosphotransfer events involving CheY carried out over a range of pH conditions elucidated several features of the phosphotransfer mechanism. Using tryptophan fluorescence intensity measurements as a monitor of phosphorylation, we showed that phosphorylation using small molecule phosphodonors occurred by fast(More)
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