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Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and(More)
We have studied the F-actin network in cycling suspension culture cells of carrot (Daucus carota L.) using rhodaminyl lysine phallotoxin (RLP). In addition to conventional fixation with formaldehyde, we have used two different nonfixation methods before adding RLP: extracting cells in a stabilizing buffer; inducing transient pores in the plasma membrane(More)
Efficient gene transfer to muscle stem cells (satellite cells) has not been achieved despite broad transduction of skeletal muscle by systemically administered adeno-associated virus serotype 2/9 (AAV-9) in mice. We hypothesized that cellular migration during fetal development would make satellite cells accessible for gene transfer following in utero(More)
Cortical microtubule arrays in meristematic and differentiated cortical cells from root tips of Raphanus sativus were studied using both immunofluorescence and dry cleaving. Length, density and orientation of the cortical microtubules were measured. Between individual, non-dividing cells of the meristematic zone the mean microtubule length varied from 0.9(More)
To visualize the localization of cell surface constituents in relation to the plasma membrane-associated filament network, we developed a method based on a combination of immunogold labeling and dry-cleaving. For labeling we used trinitrophenyl-derivatized ligand, anti-TNP antibodies, and protein A-coated colloidal gold. Dry-cleaving (Mesland, D. A. M., H.(More)
Because two division planes form at right angles, male meiosis in higher plants provides striking examples of both division control and spatial programming. To investigate these processes we have stained microtubules and actin filaments during male mei-osis in the eggplant. Our results indicate the following. (1) That microtubules and their nucleation sites(More)
A method is described for localizing microtubules using gold-labeled antibodies in combination with anti-tubulin. Cortex cells of Equisetum hyemale are broken open while still in buffer, after initially being attached to poly-L-lysine-coated grids. Thus, the cytoplasm becomes accessible to the antibodies. After application of the antibodies, the cleaved(More)
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