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Chinese hamster primary fibroblasts derived from several embryos were treated with the carcinogens benzo(a)pyrene, 7,12-dimethylbenz(a)anthracene or N-methyl-N'-nitro-N-nitrosoguanidine. Karyotype analysis, sister chromatid exchange frequency, evidence of transformation by growth in agar, cell morphology and reaction to cytocholasin B were tested at regular(More)
The relationship of cytogenetic changes with the acquisition of an indefinite life span in vitro, the ability of cells to grow in soft agar and their tumourigenicity in syngeneic animals has been studied in control, trans-7,8-dihydrodiolbenzo(a)pyrene and 7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo(a)-pyrene-treated secondary(More)
The ability of three ultimate matabolites of benzo(a)-pyrene and of 7-bromomethylbenz(a)anthracene to induce 8-azaguanine mutants, sister-chromatid exchanges and chromosome aberrations has been investigated. 7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene was shown to be an extremely efficient inducer of both mutants and(More)
Examples of chromic and chromate salts have been examined for their effects on a cultured Chinese hamster cell line. The responses studied were cytotoxicity, mutagenesis and clastogenesis. Chromate (hexavalent chromium) salts of both high and medium water solubility were active in producing all three classes of response, whereas an insoluble chromate salt(More)
The effect of 5 unrelated chemicals with different promoting potencies on sister chromatid exchange (SCE) induction in V79 cells was investigated. Two powerful promoters--12-O-tetradecanoylphorbol-13-acetate (TPA) and anthralin; a moderate promoter--iodoacetic acid (IAA); two weak promoters--ethyl phenylpropiolate (EPP) and cantharidin--all induced similar(More)
The ability of the 12 monomethylbenz[a]anthracene isomers, following their metabolism by using the cell-mediated activation system, to induce 8-azaguanine-resistant mutants, sister-chromatid exchanges (SCEs) and chromosomal aberrations has been measured. Both the mutagenic potency and the ability of the monomethylbenz[a]anthracenes to induce SCEs correlated(More)
The relationship between ageing and transformation has been investigated by a serial study of the changes in cell-surface morphology as normal and carcinogen-treated cells progressed in culture. A progressive increase in the density of cell surface microvilli occurred in association with the adoption of a more rounded profile and concomitant increase in the(More)