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Luciferase from the Italian firefly Luciola italica: molecular cloning and expression.
The cDNA encoding the luciferase from the Italian firefly Luciola italica was cloned using reverse transcriptase-PCR and a gene-specific primer set based on the DNA sequence of Luciola mingrelica.Expand
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Generation of enhanced stability factor VIII variants by replacement of charged residues at the A2 domain interface.
Factor VIII consists of a heavy chain (A1A2B domains) and light chain (A3C1C2 domains), whereas the contiguous A1A2 domains are separate subunits in the cofactor, factor VIIIa. The intrinsicExpand
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The role of P4-P3' residues flanking Arg336 in facilitating activated protein C-catalyzed cleavage and inactivation of factor VIIIa.
INTRODUCTION Activated protein C (APC) inactivates factor VIIIa (FVIIIa) through cleavages at Arg336 in the A1 subunit and Arg562 in the A2 subunit. Proteolysis at Arg336 occurs 25-fold faster thanExpand
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Sequences Flanking Arg336 in Factor VIIIa Modulate Factor Xa-catalyzed Cleavage Rates at this Site and Cofactor Function*
Background: FXa initially activates FVIII and subsequently inactivates FVIII/FVIIIa by proteolysis at specific cleavage sites. Results: Swapping sequences flanking these sites alters the rate byExpand
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P2 and P2′ Residues Are Important for the Efficient Proteolysis of Factor VIIIa at Arg336 Catalyzed by Activated Protein C.
Activated Protein C (APC) is an anticoagulant serine protease that proteolytically inactivates the coagulation cofactors, factors (F) Va and VIIIa. FVIIIa is a non-covalent heterotrimer consisting ofExpand
Cloning, Sequencing, and Characterization of Luciola italica Luciferase
ACKNOWLEDGEMENTS First of all, I would like to thank Dr. Bruce R. Branchini for introducing me to this project and advising me through it. I would also like to thank everyone in the lab includingExpand