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Luciferase from the Italian firefly Luciola italica: molecular cloning and expression.
- B. Branchini, Tara L. Southworth, Jennifer P. DeAngelis, A. Roda, E. Michelini
- Biology, Medicine
- Comparative biochemistry and physiology. Part B…
- 1 October 2006
The cDNA encoding the luciferase from the Italian firefly Luciola italica was cloned using reverse transcriptase-PCR and a gene-specific primer set based on the DNA sequence of Luciola mingrelica.… Expand
Generation of enhanced stability factor VIII variants by replacement of charged residues at the A2 domain interface.
Factor VIII consists of a heavy chain (A1A2B domains) and light chain (A3C1C2 domains), whereas the contiguous A1A2 domains are separate subunits in the cofactor, factor VIIIa. The intrinsic… Expand
The role of P4-P3' residues flanking Arg336 in facilitating activated protein C-catalyzed cleavage and inactivation of factor VIIIa.
- Jennifer P. DeAngelis, F. Varfaj, H. Wakabayashi, P. Fay
- Chemistry, Medicine
- Thrombosis research
- 1 November 2011
INTRODUCTION Activated protein C (APC) inactivates factor VIIIa (FVIIIa) through cleavages at Arg336 in the A1 subunit and Arg562 in the A2 subunit. Proteolysis at Arg336 occurs 25-fold faster than… Expand
Sequences Flanking Arg336 in Factor VIIIa Modulate Factor Xa-catalyzed Cleavage Rates at this Site and Cofactor Function*
- Jennifer P. DeAngelis, H. Wakabayashi, P. Fay
- Chemistry, Medicine
- The Journal of Biological Chemistry
- 12 March 2012
Background: FXa initially activates FVIII and subsequently inactivates FVIII/FVIIIa by proteolysis at specific cleavage sites. Results: Swapping sequences flanking these sites alters the rate by… Expand
P2 and P2′ Residues Are Important for the Efficient Proteolysis of Factor VIIIa at Arg336 Catalyzed by Activated Protein C.
Activated Protein C (APC) is an anticoagulant serine protease that proteolytically inactivates the coagulation cofactors, factors (F) Va and VIIIa. FVIIIa is a non-covalent heterotrimer consisting of… Expand
Cloning, Sequencing, and Characterization of Luciola italica Luciferase
- Jennifer P. DeAngelis
ACKNOWLEDGEMENTS First of all, I would like to thank Dr. Bruce R. Branchini for introducing me to this project and advising me through it. I would also like to thank everyone in the lab including… Expand
charged residues at the A2 domain interface Generation of enhanced stability factor VIII variants by replacement of