Jennifer L Keynton

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Cell-to-cell contact and polarisation of epithelial cells involve a major reorganisation of the microtubules and centrosomal components. The radial microtubule organisation is lost and an apico-basal array develops that is no longer anchored at the centrosome. This involves not only the relocation of microtubules but also of centrosomal anchoring proteins(More)
Apico-basal polarisation of epithelial cells involves a dramatic reorganisation of the microtubule cytoskeleton. The classic radial array of microtubules focused on a centrally located centrosome typical of many animal cells is lost or greatly reduced and a non-centrosomal apico-basal array develops. The molecules and mechanisms responsible for the assembly(More)
Microtubule end-binding (EB) proteins influence microtubule dynamic instability, a process that is essential for microtubule reorganisation during apico-basal epithelial differentiation. Here, we establish for the first time that expression of EB2, but not that of EB1, is crucial for initial microtubule reorganisation during apico-basal epithelial(More)
Initially identified in DNA damage repair, ATM-interactor (ATMIN) further functions as a transcriptional regulator of lung morphogenesis. Here we analyse three mouse mutants, Atmin(gpg6/gpg6), Atmin(H210Q/H210Q) and Dynll1(GT/GT), revealing how ATMIN and its transcriptional target dynein light chain LC8-type 1 (DYNLL1) are required for normal lung(More)
During mammalian development, left-right (L-R) asymmetry is established by a cilia-driven leftward fluid flow within a midline embryonic cavity called the node. This 'nodal flow' is detected by peripherally-located crown cells that each assemble a primary cilium which contain the putative Ca2+ channel PKD2. The interaction of flow and crown cell cilia(More)
Results Our expression studies indicate that a regulatory relationship might exist between FOXA2 and Pkd1l1. Furthermore, Shh and FoxA2;ShhcreER embryos display abnormal Pkd1l1 expression. Indeed, previous studies identified FOXA2 binding regions upstream of Pkd1l1, in both human and mouse. Our work has revealed the two regions to be non-homologous, but(More)
In mammals, left-right symmetry is broken during development by motile cilia-driven fluid flow in the embryonic node. How this ‘nodal flow’ is sensed remains the subject of debate. In kidney, fluid flow/membrane stress is sensed by polycystin protein complexes composed of Pkd1 and Pkd2 which reside in primary cilia. Pkd2 also plays a role in determining(More)
Motile cilia, in the embryonic node, drive a leftward fluid flow (termed nodal flow) that establishes the left-right axis. We identified lrm5 in a genetic screen for mouse leftright patterning mutants the embryos exhibited disturbed situs. Mapping and sequencing revealed a novel mutation in the axonemal dynein heavy chain locus Dnah11: loss of function(More)
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