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Enterohemorrhagic Escherichia coli Reduces Mucus and Intermicrovillar Bridges in Human Stem Cell-Derived Colonoids
Background & Aims Enterohemorrhagic Escherichia coli (EHEC) causes over 70,000 episodes of foodborne diarrhea annually in the United States. The early sequence of events that precede life-threateningExpand
Human Intestinal Enteroids: a New Model To Study Human Rotavirus Infection, Host Restriction, and Pathophysiology
TLDR
This research establishes HIEs as nontransformed cell culture models to understand human intestinal physiology and pathophysiology and the epithelial response, including host restriction of gastrointestinal infections such as HRV infection. Expand
Functional Coupling of Human Microphysiology Systems: Intestine, Liver, Kidney Proximal Tubule, Blood-Brain Barrier and Skeletal Muscle
TLDR
The potential of human MPS for multi-organ toxicity and absorption, distribution, metabolism and excretion (ADME), guidance for physically coupling MPS, and offer an approach to coupling Mps with distinct media and perfusion requirements are demonstrated. Expand
Human Enteroids/Colonoids and Intestinal Organoids Functionally Recapitulate Normal Intestinal Physiology and Pathophysiology*
TLDR
The physiologic properties of primary cultures developed from isolated intestinal crypts or stem cells and from inducible pluripotent stem cells are discussed, their current use in understanding diarrhea-causing host-pathogen interactions, and potential future applications. Expand
Human enteroids as an ex-vivo model of host–pathogen interactions in the gastrointestinal tract
TLDR
The recent progress in establishing human enteroids as a model of infectious diarrheal diseases such as cholera, rotavirus, and enterohemorrhagic Escherichia coli is discussed, and use of the enteroids to determine ways to correct the diarrhea-induced ion transport abnormalities via drug therapy. Expand
Demonstration of Starter Unit Interprotein Transfer from a Fatty Acid Synthase to a Multidomain, Nonreducing Polyketide Synthase
TLDR
The pathway for substrate transacylation between a fungal type I fatty acid synthase and a nonreducing polyketide synthase was determined by in vitro reconstitution of dissected domains by exploiting coenzyme A selectivity in the FAS acyl carrier protein. Expand
Corrigendum: Functional Coupling of Human Microphysiology Systems: Intestine, Liver, Kidney Proximal Tubule, Blood-Brain Barrier and Skeletal Muscle
TLDR
The potential of human MPS for multi-organ toxicity and absorption, distribution, metabolism and excretion (ADME), guidance for physically coupling MPS, and offer an approach to coupling Mps with distinct media and perfusion requirements are demonstrated. Expand
Human mini-guts: new insights into intestinal physiology and host–pathogen interactions
TLDR
The human enteroid model enables specific functional studies of secretion and absorption in each intestinal segment as well as observations of the earliest molecular events that occur during enteric infections. Expand
Molecular Basis and Differentiation-Associated Alterations of Anion Secretion in Human Duodenal Enteroid Monolayers
TLDR
Enteroid differentiation is associated with significant alterations in the expression of several ion transporters and carbonic anhydrase isoforms, leading to a reduced but preserved anion secretory phenotype owing to markedly reduced Cl- secretion but no significant change in HCO3- secretion. Expand
Serine Protease EspP from Enterohemorrhagic Escherichia Coli Is Sufficient to Induce Shiga Toxin Macropinocytosis in Intestinal Epithelium
TLDR
The role of type 3 secretion in actin turnover occurring during toxin macropinocytosis is investigated, which could suggest new potential therapeutic targets for Shiga toxin-induced disease. Expand
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