Learn More
Our laboratory has shown that classical synapses and synaptic proteins are associated with Type III cells. Yet it is generally accepted that Type II cells transduce bitter, sweet and umami stimuli. No classical synapses, however, have been found associated with Type II cells. Recent studies indicate that the ionotropic purinergic receptors P2X2/P2X3 are(More)
One of the challenges of manipulating genes in primary cells is that the cells have a finite proliferation capacity. This, combined with the lower gene targeting efficiency of somatic cells, makes identification of targeted clones very difficult. The objective of this study was to establish a system that allows porcine foetal fibroblasts to reach their(More)
When lens epithelium is cultured with retina-conditioned medium, many of the cells undergo fibre differentiation within 6-10 days. Here we report the temporal sequence of structural events that characterize this change in an organ culture model system. Within an hour of exposure to conditioned medium, some cells withdrew from the epithelial monolayer and(More)
Lens epithelial explants grown in retina-conditioned medium (RCM) undergo structural and molecular changes characteristic of fibre differentiation in the intact lens. We suggest that in vivo neural retina releases a fibre differentiation factor (FDF) that interacts with equatorial lens epithelial cells and stimulates them to undergo fibre cell(More)
  • 1