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OBJECTIVE To evaluate the impact of female smoking on early embryo development in vitro using a time-lapse system. DESIGN Retrospective analysis of prospectively collected database. SETTING Assisted reproductive technology (ART) program. PATIENT(S) A total of 135 infertile couples undergoing IVF-intracytoplasmic sperm injection (ICSI) cycle. … (More)
PURPOSE The purpose of our study was to use time-lapse in order to evaluate the impact of sperm origin (fresh ejaculate or surgically retrieved) on embryo morphokinetic parameters and clinical outcome in intracytoplasmic sperm injection (ICSI) cycles. METHODS This retrospective monocentric study was conducted in 485 unselected couples undergoing 604 ICSI… (More)
Among all the strategies available in order to improve success rates in IVF cycles, a lot of work has been done on embryo culture conditions and embryo quality evaluation. Most IVF centres use conventional incubators and select embryo according to punctual morphological evaluation, but this strategy has several limitations. Recently developed commercial… (More)
OBJECTIVE To study the performance of a previously published implantation prediction model based on morphokinetics in a different setting, in an unselected population and with various embryo transfer strategies. DESIGN Retrospective monocentric study. SETTING University-based assisted reproduction technology (ART) center. PATIENT(S) 450 unselected… (More)
Time-lapse analysis of tripronucleated zygotes obtained in ICSI cycles showed that 75.4% cleaved into embryos. These embryos subsequently demonstrated slower developmental kinetics than normally fertilized embryos.
Assisted Reproductive Techniques (ART) separating oocytes in sibling oocytes treated either by conventional IVF or ICSI is called mid-IVF/ICSI. We sum up here 487 attempts of this kind from six French ART centers. The mid-IVF/ICSI technique was performed in 5.6% of cases. The fertilization rate by micro-injected oocytes was significantly higher (P<0.01)… (More)
PURPOSE Despite controversy regarding its clinical value, male fertility investigation mainly relies on semen analysis. Even though reference guidelines are available, manual sperm analysis still suffers from analytical variability, thus questioning the interest of automated sperm analysis systems. The aim of this study is to compared automated computerized… (More)