Nucleocytoplasmic transport is mediated by nuclear pore complexes (NPCs) embedded in the nuclear envelope. About 30 different proteins (nucleoporins, nups) arrange around a central eightfold rotational axis to build the modular NPC. Nup188 and Nup192 are related and evolutionary conserved, large nucleoporins that are part of the NPC scaffold. Here we… (More)
Soluble karyopherins of the importin-β (impβ) family use RanGTP to transport cargos directionally through the nuclear pore complex (NPC). Whether impβ or RanGTP regulate the permeability of the NPC itself has been unknown. In this study, we identify a stable pool of impβ at the NPC. A subpopulation of this pool is rapidly turned-over by RanGTP, likely at… (More)
In this paper, we investigate how system area networks can deal with transient and permanent network failures. We design and implement a firmware–level retransmission scheme to tolerate transient failures and an on–demand network mapping scheme to deal with permanent failures. Both schemes are transparent to applications and are conceptually simple and… (More)
The organization and biophysical properties of the cytosol implicitly govern molecular interactions within cells. However, little is known about mechanisms by which cells regulate cytosolic properties and intracellular diffusion rates. Here, we demonstrate that the intracellular environment of budding yeast undertakes a startling transition upon glucose… (More)
The panel includes scholars from three countries. It includes a blend of graduate students and faculty spanning the assistant, associate and full professor level. It is also diverse in terms of discipline, representing scholars working in departments of information studies, business communications, computer science, history of science, and science policy.