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Echovirus type 30 (E30) (genus, Enterovirus; family, Picornaviridae) has caused large outbreaks of aseptic meningitis in many regions of the world in the last 40 years. U.S. enterovirus surveillance data for the period 1961 to 1998 indicated that the annual proportion of E30 isolations relative to total enterovirus isolations has fluctuated widely, from a(More)
The present study describes an improved fluorescent recovery after photobleaching (FRAP) technique, which has been successfully used to quantify actin dynamics within individual fibers. Chondrocytes were transfected with an eGFP-actin plasmid and cultured on glass coverslips. In cells expressing eGFP-actin, confocal microscopy was used to bleach 3 x 1(More)
Funded by the contributions of over 30 states and the US Federal Highway Administration, the American Association of State Highway and Transportation Officials (AASHTO) is nearing completion of a three-year project to develop a pair of related systems for bridge load rating and design, known as Virtis™ and Opis™, respectively. A consulting team led by(More)
Cytoskeleton is formed by three main types of filaments: microfilaments, microtubules and intermediate filaments. It is a dynamic structure which allows the cell to move, to keep its shape and to divide during mitosis. It also facilitates the intracellular transport of vesicles and organelles. The study of protein dynamics in vivo is now possible since the(More)
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