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The therapeutic potential of Wnt proteins has long been recognized but challenges associated with in vivo stability and delivery have hindered their development as drug candidates. By exploiting the hydrophobic nature of the protein we provide evidence that exogenous Wnt3a can be delivered in vivo if it is associated with a lipid vesicle. Recombinant Wnt3a(More)
Early luteolytic changes in the plasma membrane of luteal cells were examined in the rat. Treatment with prostaglandin F2 alpha in vivo caused a rapid transient increase in superoxide radical formation and a decrease in fluidity in plasma membrane samples prepared from luteinized rat ovaries. These alterations preceded detection of a significant fall in(More)
The function of the corpus luteum (CL) is a key element in many reproductive processes including ovulation, length of the estrous cycle, recognition of pregnancy and embryo survival in all mammalian species. The main function of the CL is to produce progesterone which acts on its tissues to prepare them for successful pregnancy. The CL is controlled by(More)
In this study we examined the mechanism of corpus luteum (CL) regression by measuring changes in expression of prostaglandin G/H synthase-1 (PGHS-1) and -2 (PGHS-2) in day 4 CL and inducible heat shock protein 70 (HSP-70) in day 4 and day 9 CL of immature superovulated rats. The rats were superovulated and treated with 500 microg of prostaglandin F2alpha(More)
Drosophila were examined to see if there is an increase in damage associated with free radical activity in older flies. The levels of superoxide radical and of lipid peroxides were higher in membrane samples from older flies. Also, in older Drosophila there was a significant decrease in membrane fluidity, as determined by fluorescence polarization, and an(More)
The free radical theory of aging was examined by measuring the formation of superoxide radical (SOR) and the level of lipid peroxides in various tissues of the aging rat. A significant increase in SOR production was seen in mitochondria prepared from the brain and the heart as rats aged. An elevation in the level of lipid peroxidation was also found in(More)
Antioxidants were used to investigate the role of free radicals in control of luteal steroidogenesis. Corpora lutea from pseudopregnant rats were enzymatically dispersed, the cells were incubated with antioxidants, and progesterone production was measured. Addition of the antioxidants nordihydroguaiaretic acid (NDGA), butylated hydroxytoluene (BHT), and the(More)
The arteriovenous difference in the concentration of prostaglandin F2alpha (PGF2alpha) across the brain of the anestrous sheep was measured before and during the induction of luteinizing hormone (LH) secretion with estradiol-17beta (E2-17beta). The results indicate that (a) the brain is a significant source of PGF2alpha, (b) the release of PGF2alpha from(More)
The ability of the superoxide radical (SOR) generated by xanthine oxidase to activate phospholipase A2 (PLA2) was examined in microsomes prepared from luteinized rat ovaries. Treatment of microsomes with xanthine oxidase resulted in a rapid burst in SOR formation followed by an increase in PLA2 activity. Stimulation of PLA2 activity was dose related and(More)