Jeffery A. Schloss

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The Human Microbiome Project (HMP), funded as an initiative of the NIH Roadmap for Biomedical Research (http://nihroadmap.nih.gov), is a multi-component community resource. The goals of the HMP are: (1) to take advantage of new, high-throughput technologies to characterize the human microbiome more fully by studying samples from multiple body sites from(More)
A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect(More)
A Chlamydomonas gene encodes a protein that shows sequence similarity with the β subunit of guanine nucleotide binding proteins from mammals, fruit fly and yeast. In addition to amino acid sequences similarity, each of these proteins contains a segmented repeat structure in which certain amino acids form a consensus sequence. Thus this gene product has been(More)
DNA sequencing-by-synthesis (SBS) technology, using a polymerase or ligase enzyme as its core biochemistry, has already been incorporated in several second-generation DNA sequencing systems with significant performance. Notwithstanding the substantial success of these SBS platforms, challenges continue to limit the ability to reduce the cost of sequencing a(More)
1113 years to reduce costs 100-fold, from $10.00 to $0.10 per finished base pair6. The solicitations also addressed a key issue in determining the true meaning of sequencing by cost by linking it to a measurable standard of sequence quality. The ‘gold standard’ for genomic sequencing was 99.99% accuracy with essentially no gaps7, but experience showed that(More)
Detachment of the flagella of Chlamydomonas induces a rapid accumulation of mRNAs for tubulin and other flagellar proteins. Measurement of the rate of alpha and beta tubulin RNA synthesis during flagellar regeneration shows that deflagellation elicits a rapid, 47-fold burst in tubulin RNA synthesis. The synthesis rate peaks within 10-15 rain, then declines(More)
To investigate the involvement of tubuiin gene expression in controlling cell division events in Chlamydomonas reinhardtii we have measured tubuiin mRNA levels during the cell cycle under different environmental conditions. In C. reinhardtii cells grown under the synchronizing conditions of 14 h of light followed by 10 h of darkness, mRNAs for tubuiin and(More)
ADMINISTRATIVE BUDGET NOTE: The budget shown is the requested budget and has not been adjusted to reflect any recommendations made by reviewers. If an award is planned, the costs will be calculated by Institute grants management staff based on the recommendations outlined below in the COMMITTEE BUDGET RECOMMENDATIONS section. RESUME AND SUMMARY OF(More)
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