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Most turtle species possess temperature-dependent sex determination (TSD), but genotypic sex determination (GSD) has evolved multiple times independently from the TSD ancestral condition. GSD in animals typically involves sex chromosomes, yet the sex chromosome system of only 9 out of 18 known GSD turtles has been characterized. Here, we combine comparative(More)
In species or developmental stages where the sex of an individual cannot be reliably identified through external morphology, molecular markers can provide a critical tool to study sex-specific traits that are elusive otherwise. Here we generated two sets of sex-diagnostic PCR primers for each of two focal turtle species with contrasting genotypic sex(More)
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