Jean Torreilles

Learn More
Inflammatory reaction is thought to be an important contributor to neuronal damage in neurodegenerative disorders such as Alzheimer's disease (AD), Parkinson's disease (PD), multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS) and the parkinsonism dementia complex of Guam. Among the toxic agents released in brain tissues by activated cells, we focus(More)
After the discovery of the vasodilatory functions of nitric oxide (NO), many signaling mechanisms involving NO were identified through experiments on mammals. NO activates soluble guanylyl cyclase to induce the formation of cGMP, stimulates ADP-ribosylation of GAPDH to alter cell energy production, and combines with superoxide to generate peroxynitrite. It(More)
The phagocytic activity of Mytilus galloprovincialis hemocytes is thought to be associated with NADPH-oxidase activity of the plasma membrane, thus producing superoxide anions. Few studies, however, have been devoted to nitric oxide release by these haemocytes. We investigated NO generation in M. galloprovincialis in order to understand its role in the(More)
Malondialdehyde assay is the most generally used test in the appreciation of the role of oxidative stress in disease. Malondialdehyde is one of several products formed during the radical induced decomposition of polyunsaturated fatty acids. Most often, malondialdehyde assay used its reactivity at high temperature and low pH, towards thiobarbituric acid.(More)
Peroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. It can be formed by combinaison of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine. The present article describes a competitive ELISA for the measurement of 3-nitrotyrosine contents of plasma proteins from(More)
To assess the role of superoxide (O2-) and nitric oxide (NO) in ischaemic-reperfusion-induced acute renal failure, we investigated whether an activation of the L-arginine-NO pathway contributes to ischaemia-reperfusion-induced kidney membrane peroxidation by measurement of 4-hydroxynonenal (HNE) content in anaesthetized rats submitted to acute renal(More)
Carbonyl compounds such as alpha-ketoglutarate, pyruvate, oxaloacetate, butyraldehyde, acetaldehyde or acetone react with NAD or NADP to give adducts. Binding studies of adducts to dehydrogenases are performed by means of ultraviolet differential spectroscopy, circular dichroism and spectrofluorimetry. The dehydrogenases show a high degree of binding(More)
Glutamate dehydrogenase is reversibly inhibited by the reaction of 1 mole of pyridoxal 5'-phosphate per mole of subunit of the enzyme polypeptide chain. We have shown that NAD(P) adducts as well as NMNH protect the glutamate dehydrogense against this reversible inactivation in the same way as reduced coenzymes. These data lead to the conclusion that it is(More)
The murexide (5,5'-nitrilodibarbituric acid, monoammonium salt) is an efficient scavenger for superoxide and hydroxyl radicals. When exposed to oxygen radicals, murexide is converted to a colorless alloxan derivative and its absorbance at 520 nm decreases in proportion to the radicals produced. It is used to detect these reactive oxygen species in(More)