Jean-Pierre Lefevre

Learn More
The NarI restriction enzyme recognition site, G1G2CG3CC, has been identified as a hotspot for -2 frameshift mutations induced by N-2-acetylaminofluorene (AAF) on the basis of a forward mutation assay in plasmid pBR322 in the bacterium Escherichia coli. AAF binds primarily to the C-8 position of guanine residues, and the three guanines of the NarI site are(More)
The three-dimensional solution structure of PMP-D2, a 35 amino acid peptide isolated from the insect Locusta migratoria, has been determined from two-dimensional 1H NMR spectroscopy data. The structure calculations were performed from 222 NOE-derived interproton distances and 11 dihedral angles calculated from the JHN-H alpha coupling constants, using(More)
Due to scaling laws, in microfluidic, flows are laminar. Consequently, mixing between two liquids is mainly obtained by natural diffusion which may take a long time or equivalently requires centimetre length channels. To reduce time and length for mixing, it is possible to generate chaotic-like flows either by modifying the channel geometry or by creating(More)
Direct continuous (DC), pulsed (PEF) or alternative (AC) electric fields are well-known to induce specific effects on living cells or on molecules (including DNA and proteins) and are commonly used in molecular or cellular biology, and more recently for clinical treatment. Pharmacological industries and medical research are interesting in the development of(More)
  • 1