Jean-Marc Collin

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A collection of monoclonal antibodies was raised against bovine plasminogen by immunizing mice with purified plasminogen. More than 300 positive clones were obtained in 1 fusion experiment (1300 hybrids tested). Four types of antibodies were characterized through their relative binding to plasmin and plasminogen in ELISA. About one-third was strictly(More)
Two sandwich enzyme-linked immunosorbent assays (ELISA) have been developed for quantitation of bovine milk plasminogen and plasmin. The assays used two monoclonal antibodies, one specific for plasminogen and the other specific for plasminogen plus plasmin. Plasmin concentration was obtained by subtracting the first concentration from the second. The assays(More)
This study investigates the mechanical behaviour of a carbonitrided steel, plastically graded material, during a spherical indentation test. We demonstrate that the behaviour of a carbonitrided steel can be simulated accurately with a seven layer sample, including the surface, five intermediate layers and the substrate. Moreover, for engineering design(More)
The indentation test consists in pressing an indenter on the surface of a tested material and in measuring continually the load F in function of the displacement h of the indenter. In order to analyse the indentation curve F(h) and extract material properties, one method among others uses the notion of the representative strain. The first aim of this paper(More)
Two monoclonal antibodies, 918(4) and 139(7), directed against either bovine or porcine pepsin, respectively, were retained among 365 positive hybridoma clones. These monoclonal antibodies were characterized by using both indirect and sandwich ELISA. Characterization of these monoclonal antibodies was further performed by the biospecific interaction(More)
The serum immunoglobulins obtained from one heifer vaccinated against brucellosis, and milk immunoglobulins from a cow suffering from a natural udder infection, were purified. The preparations were more than 99.5 per cent pure. The role of each immunoglobulin in the milk ring test was determined. Fractions sIgA and IgM induce the formation of a broad(More)
The ELISA assay was used to measure the plasminogen in milk. The results showed that plasminogen concentration was slightly higher than the corresponding enzymatic activity, which could be explained to the fact that several active sites of the enzyme are occupied by inhibitors or caseins. This work shows also that about 75% of plasminogen is bound to(More)
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