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Expression of recombinant alkaline phosphatase conjugates in Escherichia coli.
The methods described in this article are relative to the use of a positive cloning/screening recombinant system for the generation in Escherichia coli of foreign proteins fused to a highly activeExpand
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Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork.
A rapid, sensitive chemiluminescent enzyme immunoassay (CLEIA) for ractopamine (RAC) based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein was developed. The scFvExpand
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Cloning and Sequence Analysis of cDNAs Encoding Precursors of Sarafotoxins
Sarafotoxins (SRTXs) are 21-amino acid peptides structurally and functionally similar to endothelins (ETs). To understand how SRTXs are overproduced in venom glands of the snakes AtractaspisExpand
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BgK, a disulfide-containing sea anemone toxin blocking K+ channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein.
BgK, a sea anemone peptide consisting of 37 amino acid residues and 3 disulfide bonds, blocks voltage-gated potassium (Kv1) channels. Here, we report a method for producing tagged BgK in EscherichiaExpand
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Dual expression system suitable for high-throughput fluorescence-based screening and production of soluble proteins.
Many studies that aim to characterize the proteome structurally or functionally require the production of pure protein in a high-throughput format. We have developed a fast and flexible integratedExpand
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Notechis 11′2, a non-toxic phospholipase A2 from the venom of Notechis scutatus scutatus
Previously, we deduced the amino acid sequence of a novel phospholipase-A2-like protein (PLA2) from the nucleotide sequence of a cDNA isolated from a library prepared from the venom gland of theExpand
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Heparan sulfates targeting increases MHC class I- and MHC class II-restricted antigen presentation and CD8(+) T-cell response.
Heparan sulfates (HS) are carbohydrate moieties of HS proteoglycans (HSPGs). They often represent alternative attachment points for proteins or microorganisms targeting receptors. HSPGs, which areExpand
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Mutants with higher stability and specific activity from a single thermosensitive variant of T7 RNA polymerase.
A single strategy to select RNA polymerase from bacteriophage T7 (T7 RNAP) mutants in Escherichia coli with enhanced thermostability or enzymatic activity is described. T7 RNAP has the ability toExpand
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“Three-Fingered” Toxins from Hydrophid and Elapid Snakes: Artificial Procedures to Overproduce Wild-Type and Mutated Curaremimetic Toxins
Toxic proteins from animal venoms usually act on molecular targets that are critically involved in the function of a physiological system of a prey. With the ultimate view of designing newExpand
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