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  • Sandrine Braud, Pascal Belin, +9 authors Sylvaine Gasparini
  • Biology, Medicine
  • Protein expression and purification
  • 2004 (First Published: 1 November 2004)
  • BgK, a sea anemone peptide consisting of 37 amino acid residues and 3 disulfide bonds, blocks voltage-gated potassium (Kv1) channels. Here, we report a method for producing tagged BgK in EscherichiaContinue Reading
  • Jie-Xian Dong, Zhen-feng Li, +7 authors Hong Wang
  • Chemistry, Medicine
  • Analytica chimica acta
  • 2012 (First Published: 1 July 2012)
  • A rapid, sensitive chemiluminescent enzyme immunoassay (CLEIA) for ractopamine (RAC) based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein was developed. The scFvContinue Reading
  • Jean-Claude Boulain, Janie Dassa, +7 authors Frédéric Ducancel
  • Biology, Medicine
  • Protein engineering, design & selection : PEDS
  • 2013 (First Published: 1 November 2013)
  • A single strategy to select RNA polymerase from bacteriophage T7 (T7 RNAP) mutants in Escherichia coli with enhanced thermostability or enzymatic activity is described. T7 RNAP has the ability toContinue Reading