Jean-Christophe Toussaint

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Choline acetyltransferase (ChAT) is the key enzyme responsible for the synthesis of the neurotransmitter acetylcholine and is reduced in various central neurodegenerative diseases. From a previously selected 12.6 kb human choline acetyltransferase (hChAT) genomic clone, we have identified and characterized a promoter region of 895 bp. Sequence analysis(More)
Fusion gene constructs containing the human choline acetyltransferase 5' flanking region are stimulated by thyroid hormone (T3) in neuronal NG108-15 and NE1-115 cells but not in non neuronal COS-1 and JEG-3 cells. To identify potential T3 receptor binding elements (T3RE), chimeric plasmids containing various lengths of the 5' end of the hChAT gene linked to(More)
Choline acetyltransferase (CHAT) (EC is the biosynthetic enzyme for the neurotransmitter acetylcholine in the central and peripheral nervous systems. In this study, a human CHAT genomic clone has been isolated and partially sequenced at its 5' end. This fragment contains the first four exons with an ACG initiator codon and potential control regions(More)
Thin film alloys with perpendicular anisotropy were studied using Lorentz transmission electron microscopy (LTEM). This work focuses on the configuration of domain walls and demonstrates the suitability and accuracy of LTEM for the magnetic characterization of perpendicular magnetic anisotropy materials. Thin films of chemically ordered (L1(0)) FePd alloys(More)
—We investigated with XMCD-PEEM magnetic imaging the magnetization reversal processes of Néel caps inside Bloch walls in self-assembled, micron-sized Fe(110) dots with flux-closure magnetic state. In most cases the magnetic-dependent processes are symmetric in field, as expected. However, some dots show pronounced asymmetric behaviors. Micromagnetic(More)
The effect of chronic ethanol consumption on the cellular metabolism of benzo-a-pyrene (BaP) is investigated in human peripheral lymphocytes obtained from 6 proven alcoholic addicts and 5 healthy donors. Lymphocytes convert BaP to dihydrodiols, diol-epoxides, phenols, quinone derivatives and polyhydroxylated forms. Sulfate, glucuronide and glutathione(More)
We have produced monoclonal antibodies against alpha-bungarotoxin from Bungarus multicinctus, and controls were performed for their specificity and monoclonal character. The antibodies protected mice against the toxic effect of alpha-bungarotoxin for a few hours to six days, depending upon the chosen antibody (in comparison to a survival time of 79 min for(More)