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Proteins contained on purified COPII vesicles were analyzed by matrix-assisted laser desorption ionization mass spectrometry combined with database searching. We identified four known vesicle proteins (Erv14p, Bet1p, Emp24p, and Erv25p) and an additional nine species (Yip3p, Rer1p, Erp1p, Erp2p, Erv29p, Yif1p, Erv41p, Erv46p, and Emp47p) that had not been(More)
Nerve growth factor (NGF) induces neurite outgrowth and differentiation in a process that involves NGF binding to its receptor TrkA and endocytosis of the NGF-TrkA complex into signaling endosomes. Here, we find that biogenesis of signaling endosomes requires inactivation of Rab5 to block early endosome fusion. Expression of dominant-negative Rab5 mutants(More)
APPL1 is an effector of the small GTPase Rab5. Together, they mediate a signal transduction pathway initiated by ligand binding to cell surface receptors. Interaction with Rab5 is confined to the amino (N)-terminal region of APPL1. We report the crystal structures of human APPL1 N-terminal BAR-PH domain motif. The BAR and PH domains, together with a novel(More)
When refugees enter their new host countries, they are faced with a variety of challenges and often find themselves relying on others for help. Equipped with only basic technologies, refugees must utilize the little they have to navigate their new environments. Currently, there are no digital systems in place that ease the transition for newly arriving(More)
Cholesterol is essential to the growth and viability of cells. The metabolites of cholesterol include: steroids, oxysterols, and bile acids, all of which play important physiological functions. Cholesterol and its metabolites have been implicated in the pathogenesis of multiple human diseases, including: atherosclerosis, cancer, neurodegenerative diseases,(More)
In a dynamic hash table (DHT), peers in a network cooperate to maintain a distributed key-value storage. Each peer in a DHT is responsible for storing a subset of keys and their values. Especially in a peer-to-peer system, the peers may not be stable. New peers join and existing peers either fail or leave the system. This process, called churn, requires the(More)
Clock (Clk)1/COQ7 is a mitochondrial hydroxylase that is necessary for the biosynthesis of ubiquinone (coenzyme Q or UQ). Here, we investigate the role of Clk1 in neuroinflammation and consequentially dopaminergic (DA) neuron survival. Reduced expression of Clk1 in microglia enhanced the LPS-induced proinflammatory response and promoted aerobic glycolysis.(More)
RDF tuples are the building blocks of the semantic web. As more data are expressed as RDF tuples, storage capabilities become important. The data set will become increasingly large such that it is necessary for data to be stored across multiple machines. Data set will be partitioned into smaller subsets, each containing an incomplete picture about data(More)
Regression tests ensure that software systems retain correct-ness in their existing functionalities while the systems evolve. During development, regression tests allow the programmer to quickly identify problems. If there are any new failed tests, since those failures surfaced after new changes to the code base, we need to identify where this failure(More)