Learn More
Higher affinity for TnI explains how troponin C (TnC) carrying a causative hypertrophic cardiomyopathy mutation, TnC(A8V), sensitizes muscle cells to Ca(2+). Muscle fibers reconstituted with TnC(A8V) require ∼2.3-fold less [Ca(2+)] to achieve 50% maximum-tension compared to fibers reconstituted with wild-type TnC (TnC(WT)). Binding measurements rule out a(More)
To investigate effects of altering troponin (Tn)C Ca(2+) binding properties on rate of skeletal muscle contraction, we generated three mutant TnCs with increased or decreased Ca(2+) sensitivities. Ca(2+) binding properties of the regulatory domain of TnC within the Tn complex were characterized by following the fluorescence of an IAANS probe attached onto(More)
Cooperative activation of striated muscle by calcium is based on the movement of tropomyosin described by the steric blocking theory of muscle contraction. Presently, the Hill model stands alone in reproducing both myosin binding data and a sigmoidal-shaped curve characteristic of calcium activation (Hill TL (1983) Two elementary models for the regulation(More)
The reversal of flagellar motion (switching) results from the interaction between a switch complex of the flagellar rotor and a torque-generating stationary unit, or stator (motor unit). To explain the steeply cooperative ligand-induced switching, present models propose allosteric interactions between subunits of the rotor, but do not address the(More)
Ryan S. Lee,* Svetlana B. Tikunova,* Kristopher P. Kline, Henry G. Zot, Javier E. Hasbun, Nguyen Van Minh, Darl R. Swartz, Jack A. Rall, and Jonathan P. Davis Department of Physiology and Cell Biology, Ohio State University, Columbus, Ohio; Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, Texas; Department of(More)
To explain disparate decay rates of cytosolic Ca(2+) and structural changes in the thin filaments during a twitch, we model the time course of Ca(2+)-bound troponin (Tn) resulting from the free Ca(2+) transient of fast skeletal muscle. In fibers stretched beyond overlap, the decay of Ca(2+) as measured by a change in fluo-3 fluorescence is significantly(More)