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Several clinical terminologies now utilize description logic to model the logical definitions of concepts. Recent editions of the Systematized Nomenclature of Medicine (SNOMED) have been developed using the description logic Ontylog. A significant design criterion for SNOMED is to keep concept expressions simple enough to be broadly usable by clinicians,(More)
  • Jason Weis
  • Current protocols in molecular biology
  • 2001
The purpose of amplification is to provide a reagent library that can be used many times. In this protocol, bacteria containing the recombinant clones are grown on agar plates, washed off the plates, and stored in glycerol. An amplified cosmid or plasmid library will contain one library's equivalence in 10 to 100 microl. By producing 100 microl of such a(More)
DNA fragments migrate through a linear sucrose gradient at a rate that is dependent on their size. This procedure described in this unit provides good resolution for DNA fragments 5 to 60 kb in size, and sucrose gradients are also useful for purification of bacteriophage l vector arms. Partially digested genomic DNA can be fractionated for the production of(More)
  • Jason Weis
  • Current protocols in molecular biology
  • 2001
There are two commonly used protocols for the screening of recombinant bacteria with hybridization probes. One method involves the spreading of bacteria on the surface of agar using a sterile spreader. A nitrocellulose membrane filter is then placed on top of the colonies and most of each colony is transferred to the filter. This method works well when(More)
We predict a new source of jet structure in e+ecolliding beam experiments arising from photon-photon collisions. These jets are produced with a sizeable fraction of the total observable hadronic rate and have unmistakable signatures. Observations of these jets would provide important tests of the contributions of three classes of hard scattering mechanisms,(More)
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