Jason A Davy

Learn More
We analyzed the expression of five protein kinase C (PKC) isoforms in cytosolic and membrane fractions from normal rat hepatocytes compared with those of two tumorigenic cell lines FAO and HepG2. Western blots with PKC-specific isoenzymes polyclonal antibodies provide evidences for the presence of the five isoforms alpha, beta II, delta, epsilon and zeta in(More)
The possible correlation between sialic acid content of hepatocyte plasma membranes, and their binding capacity was investigated. For that purpose, [3H] asialotransferrin binding was tested with hepatocytes from both normal and streptozotocin treated rats. The observed decrease in binding capacity was parallel to a decrease in the sialic acid content(More)
Using isoenzyme-specific antisera, five Protein Kinase Cs (PKCs) were detected in cytosol and membrane hepatocytes from normal rats: PKC alpha (80 kDa), PKC beta II (40, 50, 55, 85 kDa), PKC delta (74, 76 kDa), PKC epsilon (95 kDa), PKC zeta (65, 70 kDa). STZ-diabetes induced a lower expression of the five PKCs, a higher localization in the cytosol, a(More)
We aimed to relate the root characteristics of native plants, with their benefit from mycorrhizal colonization, to assist in developing criteria for predicting plant response to mycorrhizal fungi in revegetated soils. The response of four plant species to VA mycorrhizal fungi was measured at increasing applications of phosphorus (P). The benefit of(More)
The synthesis of three phosphoglycerols is described, one of which contains the previously unknown phosphonoglycerol headgroup. The surface tension-lowering capabilities of synthetic lung surfactant mixtures containing the PG analogs were measured on the pulsating bubble surfactometer and compared to known controls. The PG-containing mixtures exhibited(More)
This paper reports the chemical synthesis and purification of a novel phospholipase-resistant C16:0, C16:1 diether phosphonoglycerol with structural analogy to ester-linked anionic phosphatidylglycerol (PG) in endogenous pulmonary surfactant. This diether phosphonoglycerol (PG 1) is studied for phospholipase A(2) (PLA(2)) resistance and for surface activity(More)
In the present study we followed the different steps of epidermal growth factor receptor (EGF-R) endocytosis in freshly isolated rat hepatocytes. Hepatocytes exhibit two classes of surface EGF receptors consisting of approximately 5,000 high-affinity sites (Kd = 15 pM) and 166,000 low-affinity sites (Kd = 670 pM). Binding of labeled EGF to hepatocytes(More)
Hepatocytes isolated from streptozotocin treated Rats bind less asialotransferrin than hepatocytes isolated from normal rats. This decrease is parallel with a decrease in the sialic acid content. Insulin therapy restored simultaneously membrane sialic acid content and asialotransferrin binding capacity.
The calibration curves for evaluating the degree of desialylation of C1-inactivator (sialic acid content 12.5%) and haemopexin (sialic acid content 4%) have been plotted. No desialylation of either glycoprotein occurs in normal subjects. In the patients (liver damage) studied, C1-inactivator is often desialylated, whereas haemopexin is not. In a previous(More)
Isolated hepatocytes offer many advantages. Many experiments can be realized with homogeneous cells. Furthermore, if a pathological state or a nutritional deficiency are induced in the whole animals, freshly isolated hepatocytes can be used to evaluate the consequences of such treatment. Gluconeogenesis was studied, using isolated hepatocytes of high(More)