Jaroslav Ondracek

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Intracellular flow cytometry is a method of cytokine detection that allows simultaneous detection of intracellular cytokines and cell surface markers. This important method is not extensively used in pigs, in particular due to the inaccessibility of proper methodological protocols modifying comprehensive human protocols. The aim of this study was to find(More)
Developmental changes of functional ability of peripheral blood phagocytes from days 1 to 100 of life were investigated. Luminol enhanced chemiluminiscence was used to establish the ability of phagocytes to produce reactive oxygen species (ROS). Simple superoxide anion production was determined by spectrophotometrical measurement of cytochrome c. Activity(More)
The aim of this work was to evaluate ontogeny of reactive nitrogen species (RNS) production by peripheral blood phagocytes in pig. Pig fetuses (55 and 92 days of gestation) and postnatal piglets (1, 3, 8, 17, 31 and 41 days after birth) were used. RNS production was measured by fluorescent probes diaminofluorescein-diacetate (DAF-FMDA) and(More)
Preparation of theShigella flexneri 2a lipopolysaccharide labelled with32P is described. For the extraction we used a mixture of phenol and water (1∶1) at 65–69°C. Ballast substances were removed by precipitation with trichloracetic acid and by ethanol. The preparation was checked by agar electrophoresis. Ouchterlony's diffusion and immunoelectrophoresis.(More)
Brychta J., Smola J., Pipek P., Ondráček J., Bednář V., Čížek A., Brychta T. (2009): The occurrence of enterotoxigenic isolates of B. cereus in foodstuffs. Czech J. Food Sci., 27: 284–292. Enterotoxigenic Bacillus cereus was detected in a variety of meat stuffs (36), ready-to-cook products (5), and swabs (7). The bacterial colonies isolated from PEMBA agar(More)
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