Jaromir Hubalek

Rene Kizek28
Libuse Trnkova19
Ales Horna7
28Rene Kizek
19Libuse Trnkova
7Ales Horna
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Miniaturization and integration of chemical devices into modules that are dimensionally comparable with electronic chips (Lab on Chip) is nowadays developing worldwide. The aim of our work was to suggest and optimize the best conditions for fabrication of TFT sensor due to its sensitivity and low experimental deviations. New electrochemical analytical(More)
The use of fluorescent nanoparticles as probes for bioanalytical applications is a highly promising technique because fluorescence-based techniques are very sensitive. Quantum dots (QDs) seem to show the greatest promise as labels for tagging and imaging in biological systems owing to their impressive photostability, which allow long-term observations of(More)
Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on(More)
About biological affecting of flavonoids on animal organisms is known less, thus we selected flavonoids, flavanones and flavones, and their glycosides, which were examined as potential inducers of cytochrome(s) P450 when administrated by gavages into experimental male rats. The study was focused on induction of CYP1A1, the major cytochrome P450 involved in(More)
  • Sona Krizkova, Pavel Ryant, Olga Krystofova, Vojtech Adam, Michaela Galiova, Miroslava Beklova +10 others
  • 2008
The aim of this work is to investigate sunflower plants response on stress induced by silver(I) ions. The sunflower plants were exposed to silver(I) ions (0, 0.1, 0.5, and 1 mM) for 96 h. Primarily we aimed our attention to observation of basic physiological parameters. We found that the treated plants embodied growth depression, coloured Sensors 2008, 8(More)
The aim of this study was to describe behaviour, kinetics, time courses and limitations of the six different fully automated spectrometric methods--DPPH, TEAC, FRAP, DMPD, Free Radicals and Blue CrO5. Absorption curves were measured and absorbance maxima were found. All methods were calibrated using the standard compounds Trolox® and/or gallic acid.(More)
Routine determination of trace metals in complex media is still a difficult task for many analytical instruments. The aim of this work was to compare three electro-chemical instruments [a standard potentiostat (Autolab), a commercially available miniaturized potentiostat (PalmSens) and a homemade micropotentiostat] for easy-to-use and sensitive(More)
Metallothioneins play a key role in maintaining homeostasis of essential metals and in protecting of cells against metal toxicity as well as oxidative damaging. Excepting humans, blood levels of metallothionein have not yet been reported from any animal species. Blood plasma samples of 9 animal species were analysed by the adsorptive transfer stripping(More)
  • Ondrej Zitka, Dalibor Huska, Sona Krizkova, Vojtech Adam, Grace J. Chavis, Libuse Trnkova +3 others
  • 2007
Despite very intensive research in the synthesising of new cytostatics, cisplatin is still one of the most commonly used anticancer drugs. Therefore, an investigation of interactions of cisplatin with different biologically important amino acids, peptides and proteins is very topical. In the present paper, we utilized flow injection analysis coupled with(More)
Lactoferrin is a multifunctional protein with antimicrobial activity and others to health beneficial properties. The main aim of this work was to propose easy to use technique for lactoferrin isolation from cow colostrum samples. Primarily we utilized sodium dodecyl sulphate – polyacrylamide gel electrophoresis for isolation of lactoferrin from the real(More)