Jared E. Fein

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Mutants of Bacillus subtilis strain 168 have been isolated that are at least 90 to 95% deficient in the autolytic enzymes N-acetylmuramyl-L-alanine amidase and endo-beta-N-acetylglucosaminidase. These mutants grow at normal rates as very long chains of unseparated cells. The length of the chains is directly related to the growth rates. They are nonmotile(More)
High fermenter (volumetric) ethanol productivities (⩾80 g/lh−1) were attained in a simple single-stage continuous-stirred-tank-reactor (CSTR) employing a flocculent mutant of Zymomonas mobilis with a feed containing 100g/l glucose. Under these conditions a final ethanol concentration of 47.6 g/l was obtained, representing a maximum conversion efficiency of(More)
The minimum inhibitory concentration for some compounds frequently present in commercial wood acid hydrolysates are presented for Zymomonas mobilis ATCC 29191. All of the compounds tested caused morphological disturbances at sub-inhibitory concentrations. Extensive filamentous growth was observed in the presence of a complex wood hydrolysate fraction,(More)
Process conditions for the acid hydrolysis of pine hemicellulose and cellulose have been described which provide a biocompatible sugar solution. By using an improved strain of recombinant Escherichia coli, strain KO11, hydrolysates supplemented with yeast extract and tryptone nutrients were converted to ethanol with an efficiency of 85% to over 100% on the(More)
The transport of neutral amino acids in marine pseudomonad B-16 (ATCC 19855) has been investigated. From patterns of competitive inhibition, mutant analysis, and kinetic data, two active transport systems with overlapping substrate specificities were distinguished and characterized. One system (DAG) served glycine, D-alanine, D-serine, and(More)
A two-stage continuous-stirred-tank-reactor (CSTR) system is used to achieve high final alcohol concentrations (>100 g/l) with Zymononas mobilis ATCC 29191. By employing continuous cell recycle on the second stage CSTR of a two-stage process a high overall volumetric productivity (18.13 g/l/h) is achieved together with >100 g/l effluent alcohol.
Growth of Zymomonas mobilis ATCC 29191 and CP4 in a continuous stirred tank fermentor resulted in the selection of stable flocculating variants. Factors responsible for enhancing the system pressures selective for the morphological variants were identified. By incorporating some modifications into the design of the fermentor, it was possible to achieve(More)
Two poorly lytic, chain-forming mutants of Bacillus subtilis 168, strains FJ3 and FJ6, each 90-95% deficient in the production of N-acetylmuramoyl-l-alanine amidase and endo-beta-N-acethyglucosaminidase, grew helically under a variety of cultural condtions. The structures formed ranged in complexity from double-standed helices to complex aggregates of(More)
Autolytic enzymes were found to be required for flagellar morphogenesis in Bacillus subtilis 168 and Bacillus licheniformis 6346. Two previously characterized, poorly lytic, chain-forming mutants of B. subtilis 168, strains FJ3 (temperature conditional) and FJ6, each 90 to 95% deficient in the production of N-acetylmuramyl-L-alanine amidase and(More)