Jared Cardon

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The purpose of this study was to create a monoculture biofilm of a clinical isolate of Enterococcus faecalis and to determine susceptibility against four antimicrobial irrigants. Biofilms were subjected to 1-, 3-, and 5-minute exposures to one of the following irrigants: 6% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate (CHX) or one of two new(More)
Techniques are described for studying the labeling of ADP and ATP bound to the ATP synthase complex of beef heart submitochondrial particles catalyzing oxidative phosphorylation. These suffice for measurements of bound nucleotides during the time required for a single turnover, during steady state net ATP synthesis, or under quasiequilibrium conditions of(More)
This study determined if any of six endodontic solutions would have a softening effect on resorcinol-formalin paste in extracted teeth, and if there were any differences in the solvent action between these solutions. Forty-nine single-rooted extracted teeth were decoronated 2 mm coronal to the CEJ, and the roots sectioned apically to a standard length of 15(More)
An Alternate Reality Game (ARG) is an interactive storygame hybrid whose core mechanics include collaborative problem solving and storytelling. ARGs are also participatory experiences, because game designers dynamically adjust content in response to players' actions as game play progresses. What if the participatory process was extended during the design(More)
The binding of reduced nicotinamide adenine dinucleotide phosphate (NADPH) to chicken liver fatty acid synthase has been studied by using both fluorescence titrations and the direct binding method of forced dialysis. Four apparently identical sites are found per enzyme molecule, with an intrinsic dissociation constant of 0.6 microM at pH 7.0, 23 degrees C.(More)
The acyl-binding sites of avian fatty acid synthase have been further characterized using the fluorescent acetyl-CoA analog, beta-[N-(7-nitro-2,1,3-benzoxadiazol-4-yl]alanyl coenzyme A (NBDA-CoA). Binding to nonsulfhydryl sites has been studied by treatment with neutralized hydroxylamine to remove NBDA bound in a thioester linkage. The binding of NBDA to(More)
An approach previously published from this laboratory for measurement of the rate of dissociation of ATP from its complex with myosin has been carefully evaluated. The procedure has been found valid, and the off constant (21 degrees C, 1=0.21 M, pH 7.0) is 1x 10(-4)s(-1). Other data for the rate of ATP binding give a Kd for myosin ATP of 6x10(-11) M.(More)
The evidence for equivalent catalytic sites in tetrameric glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle has been re-examined and found to be insufficient to exclude alternating or reciprocating sites models. Using a column centrifugation technique, lower limits have been set on the rates of binding and release of coenzyme, and on the ratio of(More)
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