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An increasingly common component of studies in synthetic and systems biology is analysis of dynamics of gene expression at the single-cell level, a context that is heavily dependent on the use of time-lapse movies. Extracting quantitative data on the single-cell temporal dynamics from such movies remains a major challenge. Here, we describe novel methods(More)
A prerequisite for the mechanistic simulation of a biochemical system is detailed knowledge of its kinetic parameters. Despite recent experimental advances, the estimation of unknown parameter values from observed data is still a bottleneck for obtaining accurate simulation results. Many methods exist for parameter estimation in deterministic biochemical(More)
Advances in biology and engineering have enabled the reprogramming of cells with well-defined functions, leading to the emergence of synthetic biology. Early successes in this nascent field suggest its potential to impact diverse areas. Here, we examine the feasibility of engineering circuits for cell-based computation. We illustrate the basic concepts by(More)
Syndromic surveillance has, so far, considered only simple models for Bayesian inference. This paper details the methodology for a serious, scalable solution to the problem of combining symptom data from a network of U.S. hospitals for early detection of disease outbreaks. The approach requires high-end Bayesian modeling and significant computation, but the(More)
We explore how the big-three computing paradigms—symmetric multi-processor (SMP), graphical processing units (GPUs), and cluster computing—can together be brought to bear on large-data Gaussian processes (GP) regression problems via a careful implementation of a newly developed local approximation scheme. Our methodological contribution focuses primarily on(More)
We describe a strategy for Markov chain Monte Carlo analysis of non-linear, non-Gaussian state-space models involving batch analysis for inference on dynamic, latent state variables and fixed model parameters. The key innovation is a Metropolis-Hastings method for the time series of state variables based on sequential approximation of filtering and(More)
Management policies for disease outbreaks balance the expected morbidity and mortality costs versus the cost of intervention policies. We present a methodology for dynamic determination of optimal policies in a stochastic compartmental model with parameter uncertainty. Our approach is to first carry out sequential Bayesian estimation of outbreak parameters(More)
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