Learn More
It was found that the soil-dwelling fungus Rhizoctonia praticola 93A was capable to produce laccase in submerged cultures. Effects of culture conditions on the enzyme biosynthesis in shaken flask and aerated bioreactor cultures were evaluated to improve the yields of the process. Production of extracellular laccase was considerably intensified by the(More)
The cell wall material from fruiting bodies of Laetiporus sulphureus has been suggested as a new alternative to mutan for the mutanase induction in Trichoderma harzianum. Structural analyses revealed that the alkali-soluble wall fraction from this polypore fungus contained 56.3% of (1-->3)-linked alpha-glucans. When the strain T. harzianum F-340 was grown(More)
The strain Streptococcus sobrinus CCUG 21020 was found to produce water-insoluble and adhesive mutan. The factors influencing both stages of the mutan production, i.e. streptococcal cultures and glucan synthesis in post-culture supernatants were standardized. The application of optimized process parameters for mutan production on a larger scale made it(More)
PURPOSE OF WORK To show biological activity of carboxymethylated α-(1 → 3)-D-glucans isolated from the selected macromycetes fungi on human tumor and normal cells. Water-insoluble, alkali-soluble polysaccharides (WIP) were isolated from fruiting bodies of four macromycetes fungi: Lentinus edodes, Pleurotus ostreatus, Piptoporus betulinus and Laetiporus(More)
The activities of cellulases and xylanase were determined in laboratory cultures of Aspergillus terreus F-413 performed on natural and chemically or hydrothermally pretreated oak sawdust. The best stimulation effects were obtained in the cultures containing sawdust treated with dioxane, sodium hydroxide, or phosphoric acid. Moreover, the sawdust(More)
Alpha-D-glucuronidase was isolated from the culture filtrate of Phlebia radiata grown on wheat bran and purified to homogeneity by chromatographic methods. The final enzymic preparation was purified 65-fold with an activity yield of 58%; it showed a high level of specific activity (over 23,000 nkat/mg protein). The molecular and hydrolytic properties of the(More)
Of the three cariogenic streptococci grown in four various culture media, the strain Streptococcus mutans 20,381 was found to produce large amounts of extracellular glucosyltransferase and water-insoluble, adhesive exopolysaccharide when grown in batch culture on brain-heart infusion broth. Methylation and nuclear magnetic resonance analyses revealed that(More)
Dental plaque is a highly diverse biofilm, which has an important function in maintenance of oral and systemic health but in some conditions becomes a cause of oral diseases. In addition to mechanical plaque removal, current methods of dental plaque control involve the use of chemical agents against biofilm pathogens, which however, given the complexity of(More)
The chemical structure of a water insoluble α-glucan isolated from the cell wall of Aspergillus wentii was described on the basis of total acid hydrolysis, methylation analysis, and 1D and 2D NMR studies (TOCSY, DQF-COSY, NOESY and HSQC) as well as other instrumental techniques. It was established that the analyzed preparation contained a linear polymer(More)
Laetiporus sulphureus is an edible wood-rotting basidiomycete fungus whose fruiting bodies contain substances with verified therapeutic evidences and large amounts of α-(1 → 3)-glucan which is used as an effective inducer of microbial α-(1 → 3)-glucanases. However, production of mature fruiting bodies of this species under artificially controlled conditions(More)