Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'.
- Janelle R. Thompson, L. Marcelino, M. Polz
- BiologyNucleic Acids Research
- 1 May 2002
A method to eliminate heteroduplexes from mixed- template PCR products by subjecting them to 'reconditioning PCR', a low cycle number re-amplification of a 10-fold diluted mixed-template PCR product is developed.
Diversity and Dynamics of a North Atlantic Coastal Vibrio Community
- Janelle R. Thompson, M. Randa, L. Marcelino, A. Tomita‐Mitchell, Eelin Lim, M. Polz
- Environmental ScienceApplied and Environmental Microbiology
- 1 July 2004
Temperature is a good predictor of the occurrence of closely related vibrios but that considerable microdiversity of unknown significance coexists within this trend, suggesting distinct warm-water and year-round populations.
Genotypic Diversity Within a Natural Coastal Bacterioplankton Population
- Janelle R. Thompson, S. Pacocha, M. Polz
- Environmental Science, BiologyScience
- 25 February 2005
This work shows that this group of coastal bacterioplankton consists of at least a thousand distinct genotypes, each occurring at extremely low environmental concentrations (on average less than one cell per milliliter), and shows extensive allelic diversity and size variation.
Microbes in the coral holobiont: partners through evolution, development, and ecological interactions
- Janelle R. Thompson, H. Rivera, Collin J Closek, M. Medina
- Environmental Science, BiologyFrontiers in Cellular and Infection Microbiology
- 7 January 2015
Elucidating the role that microorganisms play in the structure and function of the holobiont is essential for understanding how corals maintain homeostasis and acclimate to changing environmental conditions.
Characterization of Metagenomes in Urban Aquatic Compartments Reveals High Prevalence of Clinically Relevant Antibiotic Resistance Genes in Wastewaters
- Charmaine Ng, M. Tay, K. Gin
- BiologyFrontiers in Microbiology
- 16 November 2017
Wastewaters from clinical isolation wards, in particular, had a exceedingly high levels of blaKPC-2 genes, encoding for carbapenem resistance, which suggests that hospital wastewaters derived from clinical specialty wards are hotspots for the spread of AMR.
Extensive Variation in Intracellular Symbiont Community Composition among Members of a Single Population of the Wood-Boring Bivalve Lyrodus pedicellatus (Bivalvia: Teredinidae)
- Y. Luyten, Janelle R. Thompson, W. Morrill, M. Polz, D. Distel
- BiologyApplied and Environmental Microbiology
- 1 January 2006
ABSTRACT Shipworms (wood-boring bivalves of the family Teredinidae) harbor in their gills intracellular bacterial symbionts thought to produce enzymes that enable the host to consume cellulose as its…
Secondary metabolite gene expression and interplay of bacterial functions in a tropical freshwater cyanobacterial bloom
- K. Penn, Jia Wang, S. Fernando, Janelle R. Thompson
- Environmental Science, BiologyThe ISME Journal
- 20 March 2014
Cyanobacterial harmful algal blooms (cyanoHABs) appear to be increasing in frequency on a global scale. The Cyanobacteria in blooms can produce toxic secondary metabolites that make freshwater…
Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities
- Boonfei Tan, Charmaine Ng, J. Nshimyimana, Lay Leng Loh, K. Gin, Janelle R. Thompson
- Environmental Science, BiologyFrontiers in Microbiology
- 1 September 2015
Water quality is an emergent property of a complex system comprised of interacting microbial populations and introduced microbial and chemical contaminants. Studies leveraging next-generation…
Dynamics of Vibrio Populations and Their Role in Environmental Nutrient Cycling
- Janelle R. Thompson, M. Polz
- Biology, Environmental Science
- 2006
Environmental genomic approaches to explore the metabolic diversity associated with phylogenetic clades can shed light on how widespread certain features are among the Vibrionaceae and whether vibrios are capable of as-yet- undiscovered metabolic transformations.
Accurately quantifying low-abundant targets amid similar sequences by revealing hidden correlations in oligonucleotide microarray data
- L. Marcelino, V. Backman, M. Polz
- BiologyProceedings of the National Academy of Sciences
- 12 September 2006
A general method to accurately identify low-abundant targets in systems containing complex mixtures of homologous targets by combining an analytical predictor of nonspecific probe–target interactions (cross-hybridization) with an optimization algorithm that iteratively deconvolutes true probe– target signal from raw signal affected by spurious contributions.
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