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Ecosystem respiration is the biotic conversion of organic carbon to carbon dioxide by all of the organisms in an ecosystem, including both consumers and primary producers. Respiration exhibits an exponential temperature dependence at the subcellular and individual levels, but at the ecosystem level respiration can be modified by many variables including(More)
We have analyzed the biophysical and developmental properties of Ca2+ and Na+ currents in C2 muscle cells, whose morphological and biochemical phenotype closely resembles differentiated skeletal muscle. Both fused and unfused C2 myocytes possessed: (1) membrane capacitance consistent with the presence of complex sarcotubular invaginations, (2)(More)
The mechanisms underlying the ontogeny of voltage-gated ion channels in muscle are unknown. Whether expression of voltage-gated channels is dependent on mitogen withdrawal and growth arrest, as is generally true for the induction of muscle-specific gene products, was investigated in the BC3H1 muscle cell line by patch-clamp techniques. Differentiated BC3H1(More)
Whole-cell and single-channel calcium currents were studied using single smooth muscle cells enzymatically-isolated from stomach of Amphiuma tridactylum and from guinea-pig aorta. These cells have a high specific resistance and can sustain calcium action potentials after suppression of potassium currents. Dialyzed Amphiuma smooth muscle cells had calcium(More)
Neurons and glia exhibit complex homeostatic interactions via shared extracellular space which can involve metabolites, inorganic ions, and neurotransmitters. Focal application of glutamate to both human and rat central nervous system astrocytes in primary culture produced a rapid, transient increase in both cytoplasmic and nuclear Ca2+. These Ca2+ waves(More)
A monoclonal antibody, mAb 1A, previously shown to recognize the dihydropyridine (DHP)-binding complex in rabbit muscle transverse tubules, inhibits voltage-sensitive calcium channel activity in a mouse muscle cell line. mAb 1A applied externally to BC3H1 muscle cells produced a concentration-dependent attenuation of the slowly-activating, DHP-sensitive,(More)
Oocytes of Xenopus laevis, after microinjection with mRNA from rat heart, display typical high-threshold calcium (Ca) whole cell currents. To prepare to study structure-function relationships of the cardiac Ca channel molecule, we examined the fidelity of expression of biophysical and pharmacological properties at the molecular level. Cell-attached gigaseal(More)
The nonfusing muscle cell line BC3H1 expresses functional Na and Ca channels similar to those found in skeletal muscle (1). We have utilized a monoclonal antibody that cross-reacts with a 45-50-kDa glycoprotein in differentiated BC3H1 myocytes but not in cardiac or neuronal cells. This antibody, MAb1223, specifically modulates 45Ca influx and slowly(More)
Atmospheric Chemistry and Physics Discussions This discussion paper is/has been under review for the journal Atmospheric Chemistry and Physics (ACP). Please refer to the corresponding final paper in ACP if available. Abstract To understand and quantify the impact of local, regional, and distant atmospheric mercury sources to rainfall mercury deposition in(More)
Atmospheric Chemistry and Physics Discussions This discussion paper is/has been under review for the journal Atmospheric Chemistry and Physics (ACP). Please refer to the corresponding final paper in ACP if available. Abstract Atmospheric deposition was measured at three sites in the Pensacola Bay watershed, Florida, between November 2004 and December 2007.(More)
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