Jana Svobodova

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Theory of equilibria, migration and dynamics of interconversion of a chiral analyte in electromigration enantioseparation systems involving a mixture of chiral selectors for the chiral recognition (separation) are proposed. The model assumes that each individual analyte-CS interaction is fast, fully independent on other interactions and the analyte can(More)
Stem cell (SC) therapy represents a promising approach to treat a wide variety of injuries, inherited diseases, or acquired SC deficiencies. One of the major problems associated with SC therapy remains the absence of a suitable matrix for SC growth and transfer. We describe here the growth and metabolic characteristics of mouse limbal stem cells (LSCs) and(More)
rect answers) workers. Most correct answers about knowledge of human infection by the HPAI virus also came from urban/periurban respondents. Forty percent of respondents who would not eat AI-infected chicken cited religious prohibition to eating dead animals. Seven respondents did not believe AI exists at all and viewed the outbreak situation as a(More)
Inhibition of apoptosis by the ligands of the aryl hydrocarbon receptor (AhR) has been proposed to play a role in their tumor promoting effects on liver parenchymal cells. However, little is presently known about the impact of toxic AhR ligands, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on apoptosis in other liver cell types, such as in liver(More)
We have investigated the steady-state kinetics of ubiquinol-cytochrome c reductase using either ubiquinol-1 or -2 as the donor substrate in mitochondria from Saccharomyces cerevisiae either grown at 36 degrees C or brought from 36 to 10 degrees C at the end of the exponential phase ("thermal shock"). The fatty acid composition of the mitochondrial lipids(More)
A method for determination of membrane fluidity (microviscosity) inBacillus subtilis cytoplasmic membrane underin vivo conditions is described. The membranes were labelled with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene during the exponential phase of growth. Fluorescence anisotropy values were determined as soon as 4 min after(More)
Polycyclic aromatic hydrocarbons (PAHs) with lower molecular weight exhibit lesser genotoxicity and carcinogenicity than highly carcinogenic PAHs with a higher number of benzene rings. Nevertheless, they elicit specific effects linked with tumor promotion, such as acute inhibition of gap junctional intercellular communication (GJIC). Although inflammatory(More)
Habitat fragmentation alters many ecological processes, including trophic cascades. For example, increased predation pressure along habitat edges has often been observed in fragmented landscapes. Here, we studied how nest predation risk varies along the transition zone between grassland and mixed forest in Central Europe. Using artificial nests, we tested(More)
The thermotropic behaviour of membrane phospholipids was estimated in intact cells ofBacillus subtilis. Membrane fluidity (microviscosity) of intact cells depended markedly on the ambient temperaturo — increase in cultivation temperature led to an increase in membrane fluidity. Estimated as anisotropy of 1,6-diphenyl-1,3,5-hexatriene fluorescence, a 30 %(More)
Several recent hypotheses consider oxidative stress to be a primary constraint ensuring honesty of condition-dependent carotenoid-based signalling. The key testable difference between these hypotheses is the assumed importance of carotenoids for redox homeostasis, with carotenoids being either antioxidant, pro-oxidant or unimportant. We tested the role of(More)