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Mice with solid Meth A sarcoma in the skin received an intravenous or intralesional injection of graded doses of recombinant human tumour necrosis factor (rTNF). Local treatment caused red discolouration and necrosis of the central portion of the tumour within 24 h over a larger range of doses than intravenous treatment. Effects showed a limited dose(More)
A sensitive colorimetric microassay for determining haemolytic complement activity was devised. It is carried out in U-welled microtitre dishes covered with plastic tape, which are incubated in a waterbath and subsequently centrifuged. The supernatant is transferred to flat-bottomed microtitre dishes and haemolysis is estimated by automatic measuring of the(More)
A simple photometric assay was devised for determining classical complement pathway activity in mouse serum using sensitized rabbit erythrocytes as target cells. These cells appeared more sensitive to lysis by mouse complement than sensitized mouse and sheep erythrocytes, most probably by their ability to escape the C3b inactivator system. Advantages of the(More)
The adjuvant activities of four chemically similar, but physicochemically different nonionic surface-active agents called pluronic polyols F 68, L 31, L 101 and L 121 were studied. These four agents were tested in mice using an experimental model developed for studying the adjuvant activity of the cationic surface-active agent dimethyl dioctadecyl ammonium(More)
The effects of the polyanions, carrageenan, dextran sulphate, polyanetholesulphonate and suramin, on macrophage functions and on the complement system were studied. The function of phagosomes and lysosomes in vitro was inhibited by all polyanions but carrageenan whereas phagocytosis in vitro was enhanced by small doses of all polyanions. Interaction with(More)
The adjuvanticity of Lactobacillus brevis and Lactobacillus plantarum was the subject of this study. The latter was the better adjuvant in both delayed hypersensivity and antibody formation to sheep red blood cells. Viable L. plantarum stimulated exclusively the delayed hypersensivity, where heat-killed bacteria had an adjuvant effect on antibody formation.(More)
Hexasaccharide (HS) containing 3 U of cellobiuronic acid was isolated from Streptococcus pneumoniae type 3 capsular polysaccharide S3 and coupled to bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), or tetanus toxoid (TT). The immunogenicity of these HS-protein conjugates in BALB/c mice was studied by measuring the production of circulating(More)
The effects of corticosterone and hydrocortisone on the thymus, the pituitary--adrenal axis, delayed hypersensitivity, the corticosterone plasma level and the numbers of circulating nucleated and monocytic cells were investigated in the mouse. Short-term effects within 48 h after one or two corticoid injections and late effects 7 days after a regimen of 4(More)
Enteric infections with Gram-negative bacteria are thought to play an important part in HLA-B27-associated disease such as Reiter's syndrome and reactive arthritis. But the role of bacterial infections in HLA-B27-positive ankylosing spondylitis (AS) and acute anterior uveitis (AU) is still controversial. A special interest has recently been devoted to the(More)