Jan Seynaeve

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Strains of Saccharomyces cerevisiae (NCYC 1190, 1214 and 1364) behaved as nonflocculent in defined culture medium, as well as the strain NCYC 1214 in rich medium. Flocculation was induced by Ca2+ addition and/or by correcting the pH to a suitable value. Since the free Ca2+ concentration is pH-dependent, these two factors (total Ca2+ concentration and pH)(More)
The pH, viability and flocculation during the growth of Saccharomyces cerevisiae NCYC 1214 in rich medium (YEPD) and in chemically defined medium (YNB) were compared. As the pH fell from 5.3 to 2.2, the viability fell to 20% and no flocculation was observed in YNB medium, despite the high flocculation in buffer. With the addition of 50 mM succinic acid and(More)
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