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The ordered activation of the ubiquitin protein ligase anaphase-promoting complex (APC) or cyclosome by CDC20 in metaphase and by CDH1 in telophase is essential for anaphase and for exit from mitosis, respectively. Here, we show that CDC20 can only bind to and activate the mitotically phosphorylated form of the Xenopus and the human APC in vitro. In(More)
In mammalian somatic-cell cycles, progression through the G1-phase restriction point and initiation of DNA replication are controlled by the ability of the retinoblastoma tumour-suppressor protein (pRb) family to regulate the E2F/DP transcription factors. Continuing transcription of E2F target genes beyond the G1/S transition is required for coordinating(More)
We developed a method, named GraFix, that considerably improves sample quality for structure determination by single-particle electron cryomicroscopy (cryo-EM). GraFix uses a glycerol gradient centrifugation step in which the complexes are centrifuged into an increasing concentration of a chemical fixation reagent to prevent aggregation and to stabilize(More)
Periodic activity of the anaphase-promoting complex (APC) ubiquitin ligase determines progression through multiple cell cycle transitions by targeting cell cycle regulators for destruction. At the G(1)/S transition, phosphorylation-dependent dissociation of the Cdh1-activating subunit inhibits the APC, allowing stabilization of proteins required for(More)
Ubiquitin-proteasome-mediated destruction of rate-limiting proteins is required for timely progression through the main cell cycle transitions. The anaphase-promoting complex (APC), periodically activated by the Cdh1 subunit, represents one of the major cellular ubiquitin ligases which, in Saccharomyces cerevisiae and Drosophila spp., triggers exit from(More)
In this paper, we propose a global tracking service for mobile agents, which is scalable to the Internet and accounts for security issues as well as the particularities of mobile agents (frequent changes in locations). The protocols we propose address agent impersonation, malicious location updates, as well as security issues that arise from profiling(More)
Current search engines crawl the Web, download content, and digest this content locally. For multimedia content, this involves considerable volumes of data. Furthermore, this process covers only publicly available content because content providers are concerned that they otherwise loose control over the distribution of their intellectual property. We(More)
In this paper we introduce the concept of context-aware services as an extended form of location-based services. We describe a new architecture for realizing context-aware services which has a number of key benefits. This architecture relays on mobile software agents as security-centric middleware. Our approach allows to combine disjunct databases by(More)
Current search engines crawl the Web, download content, and digest this content locally. For multimedia content, this involves considerable volumes of data. Furthermore, this process covers only publicly available content because content providers are concerned that they otherwise loose control over the distribution of their intellectual property. We(More)