Jan Panoš

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Ethanol tolerance of fourSaccharomyces cerevisiae strains characterized by different amounts of Δ5,7-sterols was tested. The individual tolerances did not correlate with the strains sterol levels. The highly and medium-accumulating strains exhibited the highest and lowest ethanol tolerances, respectively.
Improved strain ofCandida tropicalis 2838 grows on nonseparated straw hydrolyzates with no addition of vitamins and trace elements at a specific growth rate μ = 0.34 and 44 % yield coefficient (referred to reducing substances). The reducing substances in hydrolyzates contain predominantly monosaccharides (xylose, glucose, arabinose, mannose). Cells grown in(More)
In methanol-limited chemostat cultures methanol concentration >K I for growth decreased sharply the alcohol oxidase activity in yeast cells. This effect was accompanied by accumulation of riboflavin phosphate in the medium. Purified alcohol oxidase showed higherK m for methanol, change in absorbance maxima in the riboflavin area and chemical modification of(More)
The yeast strain 11Bh was studied from the aspect of qualitative and qunatitative composition of lipids formed in cells during growth on methanol, synthetic ethanol and glucose. The strain was found to form some 3% free fatty acids toward the end of the growth phase. More esterified fatty acids are formed on ethanol and glucose (2.75 and 2.86%,(More)
Corynebacterium sp. 3 B and three unidentified bacterial strains, all utilizing acetonitrile as a sole carbon and nitrogen source, were isolated from soil by the enrichment culture technique.Agrobacterium radiobacter 8/4/1, also capable of growing on acetonitrile as a sole C and N source, was obtained from a bromoxynil-metabolizing strainA. radiobacter 8/4(More)
Δ5,7 Saccharomyces cerevisiae cells growing in chemostat at a specific growth rate of 0.075/h exhibited higher ethanol tolerance measured as ethanol-induced death and anaerobic growth inhibition than the cells growing at 0.2/h, the difference being dependent on the carbon-to-nitrogen molar proportion in the medium. The observed difference in sensitivity to(More)
Detailed batch kinetics ofCandida lipolytica 4-1 onn-hexadecane for varying dispersed phase volume from 0.5 to 5% v/v is presented. All batch growth curves exhibited a linear growth region, indicating a substrate uptake limit. System productivities derived from the linear region were correlated to the dispersed phase volume. The correlation coefficient was(More)
StrainsCandida lipolytica 4-1 andCandida lipolytica K were compared in their growth and dawaxing capacities during batch growth on model gas oil. The model gas oil was composed of a mixture of even-numbered puren-alkanes (n-decane ton-dotriacontane) dissolved in dewaxed gas oil. The results show that both strains differ in their substrate specificity and in(More)
Agrobacterium radiobacter produces an extracellular polysaccharide from various carbon sources. The exopolysaccharide is produced from ethanol also in a minimal medium containing nitrate as the sole nitrogen source. On cultivation in a medium without CaCO3 only a minute amount of ethanol is converted to the exopolysaccharide. Both ethanol and nitrate in(More)