Jan H. Marcelis

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BACKGROUND Bacterial contamination of blood products is a great hazard for development of fatal transfusion reactions. Bacterial screening of platelet concentrates (PC) by aerobic and anaerobic culturing (BacT/ALERT, bioMérieux) was introduced in the Netherlands in October 2001. STUDY DESIGN AND METHODS In November 2002, a nationwide, uniform skin(More)
Bifidobacterium, Bacteroides, and Clostridium spp. isolated from the feces of 23 neonates during the first 3 months of life were identified. Of the 23 neonates, 10 were breast fed, 6 received an infant formula with iron supplement (5 mg/liter), and 7 received the formula without iron supplement (iron concentration, less than 0.5 mg/liter). The(More)
BACKGROUND AND OBJECTIVES In a previous study we established a reliable setpoint for the prevalence of bacteria in whole blood. In the present study we investigated the possible preventive effect, of diversion of the first 10 ml of a blood donation, on the bacterial contamination rate. MATERIALS AND METHODS To divert the first 10 ml of a whole-blood(More)
Growth inhibition due to iron deprivation of 118 strains of staphylococci, enterobacteriaceae, Streptococcus faecalis and Bacteroides species was studied in vitro. Apo-transferrin (Tr), apo-lactoferrin (Lf) and a synthetic iron chelator (EDDA) were used as iron binding agents. S. epidermidis was more inhibited than S. aureus. The majority of the(More)
BACKGROUND AND OBJECTIVES Dutch regulations require blood banks to check the sterility of random blood components to detect contamination during preparation. MATERIALS AND METHODS We reviewed the results of two years' testing, using standard bacteriologic methods. RESULTS Of all tested components, 0.5% were contaminated, with Staphylococcus epidermidis(More)
was done with sera put at our disposal by Dr I. Smit, (Dept. of Medical Microbiology, Free University, Amsterdam). In case of doubt biotyping with API-50E was added. Enterobacter and Serratia strains were biotyped only. A selection of the Proteus spp. strains was typed by the production of and sensitivity to proticines (Bongaerts et al., 1981). From an(More)
Lysosomotropic agents interfere with lysosome function. We studied the effects of the lysosomotropic amines: lidocaine, diphenylamine and dansylcadaverine on several functions of human polymorphonuclear leucocytes (PMN): enzyme release, phagosome-lysosome fusion, superoxide anion generation upon stimulation with opsonized bacteria, and phagocytosis and(More)
Faecal specimens from 23 infants during the first week of life were compared. Ten infants received breast milk, six received cow-milk preparation supplemented with iron (± 5 mg/l) and seven unfortified cow-milk preparation (iron concentration < 0.5 mg/l). Those on breast milk had low faecal pH, high counts of bifidobacteria and low counts(More)
To study the effect of milk supplemented with iron on neonatal gut flora, faecal specimens of ten infants receiving breast milk, six receiving a cow-milk preparation supplemented with iron (5 mg/l) and seven receiving the same product without iron supplement (iron concentration <0.5 mg/l) were examined during the first 12 weeks of life. In breast-fed(More)
The effect of iron deprivation on growth of 101 aerobic strains of gram-positive and gram-negative bacteria was studied on agar media in the presence of various concentrations of the synthetic iron chelator ethylene diamine diorthohydroxyphenyl acetic acid (EDDA) and the iron binding protein transferrin. Growth of Staphylococcus epidermidis was inhibited by(More)