Jan-Christian Hütter

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An intact preparation of adult ventricular muscle cells was incubated in substrate-free, pH-constant, anoxic Tyrode solution. The time course of metabolic changes was found to depend on the relation of cell number to incubation volume: the smaller the volume, the faster anoxic damage develops. Energy needs decline rapidly during anoxia. Yet glycolytic(More)
The effects of long-chain fatty acids on mitochondrial functions and red cell stability were studied. In albumin-containing incubation media, fatty acid distribution between the albumin-bound and the unbound fraction was estimated by calculation. When fatty acids are compared to one another on the basis of identical unbound concentrations, their(More)
Two mitochondrial subpopulations were isolated from guinea-pig heart by density gradient centrifugation. Under control conditions, both contain functionally intact mitochondria in which ischemic damage develops similarly. However, in one subpopulation adenine nucleotide content, adenine nucleotide translocase activity, oxidative phosphorylation and Ca2+(More)
It is often assumed that the release of enzymes from oxygen deficient heart tissue is due to the irreversible damage of myocardial cells. However, because of diffusion barriers and inhomogeneity of oxygen-deficient tissue this hypothesis cannot be proven in heart tissue. The question whether enzyme release may already occur during reversible injury is of(More)
Nonesterified fatty acids (NEFA), glucose and lactate are major fuels for myocardial energy production. The ratio of energy produced and oxygen consumed, which can be expressed as ATP/O ratio, is different for each substrate: e.g. 3.17 for glucose and 2.83 for palmitate. Direct measurements, however, have shown that the difference of oxygen consumption is(More)
To find a suitable index for the estimation of O2 consumption of rat hearts by use of hemodynamic parameters, isolated hearts were perfused under different working conditions. Coronary flow, arteriovenous O2 difference, cardiac output, and ventricular pressure curve were recorded and continuously fed into a computer. O2 consumption and different hemodynamic(More)
On serum precoated tissue culture dishes, isolated ventricular myocytes attach firmly during 4 hours of incubation. Since in this monoculture cells do not divide and show only minimal signs of cytoplasmic spreading, individual cells mostly lie isolated from others. However, when occasionally two cells attach in close vicinity, new cell-cell contact(More)
Myocardial fatty acid oxidation has been reported to be accompanied by an elevated O2 consumption compared with carbohydrate oxidation. The exact amount of this additional O2 consumption is controversial. Different investigators have observed an O2 wasting effect that is too large to be explained by the different ATP-to-O2 ratios of these substrates. With(More)
The dependence of (1-14C)-palmitate uptake in adult rat heart single cells on albumin was investigated. The apparent initial rate of palmitate uptake vs total palmitate concentration exhibited saturation kinetics, provided the concentration ratio of palmitate vs albumin was kept constant. However, if total palmitate was increased at constant albumin(More)
Ca2+-tolerant isolated adult heart cells can be exposed to 1 mM EGTA and then again to 1 mM CaCl2 without developing irreversible hypercontracture. Thus, they are not subject to the calcium paradox, even though they apparently become more permeable to Na+ during Ca2+-free incubation. When these cells are incubated anoxically without substrate they slowly(More)