Jan Bubeník

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Epigenetic events play an important role in tumour progression and also contribute to escape of the tumour from immune surveillance. In this study, we investigated the up-regulation of major histocompatibility complex (MHC) class I surface expression on tumour cells by epigenetic mechanisms using a murine tumour cell line expressing human E6 and E7 human(More)
Malignant conversion and subsequent in vivo selection can give rise to the cell populations that show stable expression of an immune escape phenotype, MHC class I deficient neoplasms. Deficiencies associated with the MHC class I down-regulation are either irreversible, such as beta2 microglobulin and class I heavy chain gene disabling mutations, or(More)
Therapeutic strategies based on the insertion of cytokine or other immunostimulatory genes into the genome of tumour cells followed by vaccination with the resulting, genetically modified, cytokine-producing vaccines represent a new potential prospect for the treatment of cancer patients. HPV 16 is the aetiological agent of more than 60 percent human(More)
The correlation between tumorigenicity and cell-surface adhesiveness was investigated in eight mouse fibroblastoid cell lines. Four of the lines (Mc 11-Mc 15) were derived from mouse MC-induced fibrosarcomas, two were derivatives of L cells (A9, A9HT), and two (clone 3 and clone 7 H7F4) were obtained by fusion of A9HT cells with normal diploid mouse(More)
Supernatants from Con A-stimulated rat spleen cell cultures containing T cell growth factor inhibited growth of a transplantable 3-methylcholanthrene-induced sarcoma in syngeneic mice. The tumour-inhibitory effects were dependent on the concentration of T cell growth factor and repeated injections of the supernatants.