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DNA-based immunization strategies designed to elicit cellular antitumor immunity offer an attractive alternative to protein- or peptide-based approaches. In the present study we have evaluated the feasibility of DNA vaccination for the induction of CTL reactivity to five different melanoma Ags in vitro. Cultured, monocyte-derived dendritic cells (DC) were(More)
The Ig heavy chain class switch usually occurs by breaking and rejoining DNA in the switch (S) regions, which consist of tandemly repeated sequences 5' of the constant region exons. Various studies have suggested that S DNA can also recombine with non-S sequences. To measure the frequency of such recombination events, the hybridoma cell line igm692, a(More)
This study investigated the wood surface discoloration due to simulated sunlight of tropical woods native to South America. Wood of jatoba (Hymenaea courbaril L.), massaranduba (Manilkara bidentata A. Chev.), tigerwood (Astronium graveolens Jacq.), angelim amargoso (Vatairea spp.), angelim pedra (Hymenolobium spp.) and angelim vermellho (Dinizia excelsa(More)
This study is about relationships between total extractive contents, heartwood colour and light-induced discolourations of the tropical species African Padauk (Pterocarpus soyauxii Taub) and Jatoba (Hymenaea courbaril L.). Wood surface colour was measured spectrophotometrically before and after light irradiation. Subsequently, the total extractive content(More)
The paper deals with the relationship between density and the velocity of sound wave propagation in wood. The wave velocity was measured using the heartwood of doussie (Afzelia bipindensis), merbau (Intsia bijuga), panga panga (Miletia stuhlmannii Taub.), tigerwood (Astronium graveolens Jacq.) and zebrano (Microberlinia brazzavillensis) by the two methods:(More)
We recently reported that transfected DNA inserts into the VDJ-Cmu intron much more frequently than into average DNA, and that insertion within this intron occurs preferentially into the switch region. To gain information about the mechanisms involved in DNA insertion, we sequenced the 5' and 3' junctions of typical transformants. Although the junction(More)
Stable transfection of tumor cells with IFN-alpha genes has been shown to result in abrogation of tumor establishment and induction of antitumor immunity. However, strategies suitable for the clinical application of IFN-alpha gene therapy for cancer have not been reported. In this study, we investigated two gene delivery systems capable of mediating the(More)
In the present study ectomycorrhizal development of Laccaria bicolor, Rhizopogon luteolus and Suillus bovinus associated with Scots pine (Pinus sylvestris) seedings was studied as affected by primary stand humus, secondary stand humus, podsolic sandy soil or peat in perspex growth chambers. After 9 weeks, ectomycorrhizal development with S. bovinus was(More)