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 Colonization of mycorrhizal fungi was studied in a Pinus muricata forest on the coast in California, USA, burned by a stand-replacing wildfire in October 1995. Naturally established field seedlings of P. muricata were harvested 1 yr after the fire. The species composition of the mycorrhizal fungi on these field seedlings was dominated by Rhizopogon(More)
The Ig heavy chain class switch usually occurs by breaking and rejoining DNA in the switch (S) regions, which consist of tandemly repeated sequences 5' of the constant region exons. Various studies have suggested that S DNA can also recombine with non-S sequences. To measure the frequency of such recombination events, the hybridoma cell line igm692, a(More)
This study is about relationships between total extractive contents, heartwood colour and light-induced discolourations of the tropical species African Padauk (Pterocarpus soyauxii Taub) and Jatoba (Hymenaea courbaril L.). Wood surface colour was measured spectrophotometrically before and after light irradiation. Subsequently, the total extractive content(More)
DNA-based immunization strategies designed to elicit cellular antitumor immunity offer an attractive alternative to protein- or peptide-based approaches. In the present study we have evaluated the feasibility of DNA vaccination for the induction of CTL reactivity to five different melanoma Ags in vitro. Cultured, monocyte-derived dendritic cells (DC) were(More)
We recently reported that transfected DNA inserts into the VDJ-Cmu intron much more frequently than into average DNA, and that insertion within this intron occurs preferentially into the switch region. To gain information about the mechanisms involved in DNA insertion, we sequenced the 5' and 3' junctions of typical transformants. Although the junction(More)
This study investigated the wood surface discoloration due to simulated sunlight of tropical woods native to South America. Wood of jatoba (Hymenaea courbaril L.), massaranduba (Manilkara bidentata A. Chev.), tigerwood (Astronium graveolens Jacq.), angelim amargoso (Vatairea spp.), angelim pedra (Hymenolobium spp.) and angelim vermellho (Dinizia excelsa(More)
Stable transfection of tumor cells with IFN-alpha genes has been shown to result in abrogation of tumor establishment and induction of antitumor immunity. However, strategies suitable for the clinical application of IFN-alpha gene therapy for cancer have not been reported. In this study, we investigated two gene delivery systems capable of mediating the(More)
Down-regulation of oncogene expression by antisense-based gene therapy has been extensively studied, and in some cases, therapeutic effects have been demonstrated. We have previously shown that down-regulation of HPV16 E6 and E7 gene expression inhibited HPV DNA-positive C3 mouse tumor growth. Although not all of the tumor cells were transfected by pU6E7AS(More)