Jamie A Anderson

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Keratocytes, or cornea1 fibroblasts, are the primary cell type of the cornea1 stroma. They lie between and are oriented parallel to the orthogonally arranged collagen lamellae, forming a continuous interconnecting cellular network that has been hypothesized to transmit information throughout the cornea concerning the status of the tissue (1). Under normal(More)
Conventional transmission electron microscopy (CTEM) was compared with high-voltage electron microscopy (HVEM) on 11 normal human corneas (age range, 30 weeks of gestation to 92 yr). Epithelial anchoring fibrils were noted between the basal epithelial cells and Bowman's layer (BL) as previously reported. Parallel pairs of fibers, 27.5 nm in diameter, were(More)
PURPOSE To develop an in vitro model for the study of vitamin A functions in the cornea by determining the morphologic responses to its absence and to the addition of varying concentrations in long-term (21-day) human organ culture. METHODS Donor corneas were cultured for 21 days in an air-liquid environment designed to maintain normal corneal morphology(More)
OBJECTIVES Open thoracoabdominal aneurysm repair (TAAR) is a rarely performed but a complicated and morbid procedure. This study compares the morbidity and mortality of open TAAR at high- versus low-volume hospitals. METHODS Included patients from California Office of Statewide Health Policy and Development patient discharge database who underwent an open(More)
OBJECTIVE To evaluate epithelial-stromal interactions in the healing of stromal wounds and the relationship of such interactions to regional variations in healing throughout keratotomy wounds. METHODS Ten radial keratotomy autopsy specimens were studied by using light and transmission electron microscopy. RESULTS Underneath epithelial plugs, the(More)
Long-term organ culture of human corneas offers a tool for conducting a variety of biochemical, histological, and wound-healing studies on human tissue in vitro. A requirement of any organ culture system is that cell differentiation and structural integrity of the tissue be maintained or even improved during culture The latter is particularly relevant in(More)
OBJECTIVE To determine regional differences in stromal wound healing morphologic features in long-term radial keratotomy wounds. METHODS Unsutured, semiradial, nonperforating wounds in seven monkeys and 10 human autopsy specimens obtained 3.5 to 52 months after surgery were analyzed using light, transmission, and fluorescent microscopy. RESULTS Monkeys(More)
Air/liquid organ culture of tissues with stratified epithelial layers has been shown to encourage tight packing of cells and promote cellular differentiation. In this study human corneas cultured in a air/liquid environment were compared to paired, conventionally-cultured corneas to determine if the long-term morphology could be improved. Fourteen paired(More)
Giant papillary conjunctivitis (GPC) associated with contact lens wear is believed to result from an irritative or allergic response to accumulated lens surface deposits. In a masked study, contact lenses worn short-term (2 weeks or less) or long-term (2-12 months), and obtained from patients with and without active GPC, were examined for deposited(More)
BACKGROUND Noncontact holmium:YAG laser thermal keratoplasty (Ho:YAG LTK) is a promising new technology for correction of hyperopia and astigmatism. We studied the acute histologic changes and wound healing response following Ho:YAG LTK performed with treatment parameters encompassing those used in clinical studies. METHODS We performed 10-pulse(More)