James T. Sawyer

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We previously demonstrated that a heterotypic complex of the two rat asialoglycoprotein receptor subunits was assembled during cell-free translation (Sawyer, J. T., and D. Doyle. 1990. Proc. Natl. Acad. Sci. USA. 87:4854-4858). We have characterized this system further by analyzing polypeptide interactions under both reducing and oxidizing translation(More)
For simulation practitioners, the common steps in a simulation modeling engagement are likely familiar: problem assessment, requirements specification, model building, verification, validation, and delivery of results. And for industrial engineers, it's a well-known adage that paying careful attention to <i>process</i> can help achieve better results. In(More)
Design of effective support tools for human interaction with a complex system requires models of both the human and the system. Models of the human are necessary in order to understand and evaluate the behavioral demands imposed by the system. Models of the system are necessary in order to comprehend the complexities of the work domain. The potential(More)
Simulation modeling that has been successfully used to analyze intermodal capacity issues for a wide variety of facilities. Simulation technology provides an analysis mechanism for large intermodal facilities that are difficult to duplicate with other methods due to the interaction of many variables. Automation Associates, Inc. (AAI) is a simulation(More)
SIMCAP (Simulation-based Capacity Analysis Platform) is a simulation-based software tool designed to support analysis of intermodal terminal operation, specifically with respect to track capacity and yard capacity. The application was designed with a modular, extensible software architecture with an emphasis on capturing the primary complexities that exist(More)
An intermodal rail terminal is a facility where the transfer of cargo occurs between truck and rail. The operations within these terminals involve many resources and operating rules. The ability of a terminal to respond to activity peaks that occur as a result of train arrivals and departures is critical. In order to explore how operations can be improved(More)
In the industrial engineering community, it's a well-known adage that focusing on <i>process</i> can help achieve better results. In this second of a series of papers, we'll focus on the <i>process of simulation testing</i> and outline how improving your testing process can lead to better results for your projects. We'll consider model building as a(More)
To analyze the importance of the endoplasmic reticulum oxidizing state for the folding and aggregation of newly made polypeptides, we have incubated intact HepG2 hepatoma cells in the presence of dithiothreitol. When dithiothreitol-treated cells were extracted under nondenaturing conditions immunoprecipitates of newly synthesized albumin showed a complex(More)
We have generated antibodies against synthetic peptides which represent the carboxyl terminus of either the major, or the two minor, forms of the rat hepatic lectin which recognizes galactose-terminated glycoproteins (asialoglycoproteins). The antibodies were shown to be specific for the form of the lectin containing the immunizing peptide sequence by the(More)
This presentation reviews modeling transportation systems which include port operations and the extended railroad network used by port related traffic. The case study presented encompasses the port systems and rail networks of the Pacific Northwest. A new approach for unloading and loading ships at port is introduced in this presentation. Traditionally, a(More)