James T. Sawyer

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To analyze the importance of the endoplasmic reticulum oxidizing state for the folding and aggregation of newly made polypeptides, we have incubated intact HepG2 hepatoma cells in the presence of dithiothreitol. When dithiothreitol-treated cells were extracted under nondenaturing conditions immunoprecipitates of newly synthesized albumin showed a complex(More)
We previously demonstrated that a heterotypic complex of the two rat asialoglycoprotein receptor subunits was assembled during cell-free translation (Sawyer, J. T., and D. Doyle. 1990. Proc. Natl. Acad. Sci. USA. 87:4854-4858). We have characterized this system further by analyzing polypeptide interactions under both reducing and oxidizing translation(More)
We have translated RNAs for the two rat asialoglycoprotein receptor polypeptides together in a cell-free system containing dog pancreatic microsomes and immunoprecipitated the products with antibodies that distinguish the two proteins. In this system the proteins oligomerize, as judged by their coprecipitation with either of the subunit-specific antisera.(More)
We have generated antibodies against synthetic peptides which represent the carboxyl terminus of either the major, or the two minor, forms of the rat hepatic lectin which recognizes galactose-terminated glycoproteins (asialoglycoproteins). The antibodies were shown to be specific for the form of the lectin containing the immunizing peptide sequence by the(More)
For simulation practitioners, the common steps in a simulation modeling engagement are likely familiar: problem assessment, requirements specification, model building, verification, validation, and delivery of results. And for industrial engineers, it's a well-known adage that paying careful attention to <i>process</i> can help achieve better results. In(More)
SIMCAP (Simulation-based Capacity Analysis Platform) is a simulation-based software tool designed to support analysis of intermodal terminal operation, specifically with respect to track capacity and yard capacity. The application was designed with a modular, extensible software architecture with an emphasis on capturing the primary complexities that exist(More)
Design of effective support tools for human interaction with a complex system requires models of both the human and the system. Models of the human are necessary in order to understand and evaluate the behavioral demands imposed by the system. Models of the system are necessary in order to comprehend the complexities of the work domain. The potential(More)
In the industrial engineering community, it's a well-known adage that focusing on <i>process</i> can help achieve better results. In this second of a series of papers, we'll focus on the <i>process of simulation testing</i> and outline how improving your testing process can lead to better results for your projects. We'll consider model building as a(More)