James T. Sawyer

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We previously demonstrated that a heterotypic complex of the two rat asialoglycoprotein receptor subunits was assembled during cell-free translation (Sawyer, J. T., and D. Doyle. 1990. Proc. Natl. Acad. Sci. USA. 87:4854-4858). We have characterized this system further by analyzing polypeptide interactions under both reducing and oxidizing translation(More)
To analyze the importance of the endoplasmic reticulum oxidizing state for the folding and aggregation of newly made polypeptides, we have incubated intact HepG2 hepatoma cells in the presence of dithiothreitol. When dithiothreitol-treated cells were extracted under nondenaturing conditions immunoprecipitates of newly synthesized albumin showed a complex(More)
We have generated antibodies against synthetic peptides which represent the carboxyl terminus of either the major, or the two minor, forms of the rat hepatic lectin which recognizes galactose-terminated glycoproteins (asialoglycoproteins). The antibodies were shown to be specific for the form of the lectin containing the immunizing peptide sequence by the(More)
Design of effective support tools for human interaction with a complex system requires models of both the human and the system. Models of the human are necessary in order to understand and evaluate the behavioral demands imposed by the system. Models of the system are necessary in order to comprehend the complexities of the work domain. The potential(More)
We have translated RNAs for the two rat asialoglycoprotein receptor polypeptides together in a cell-free system containing dog pancreatic microsomes and immunoprecipitated the products with antibodies that distinguish the two proteins. In this system the proteins oligomerize, as judged by their coprecipitation with either of the subunit-specific antisera.(More)
To determine changes in distribution or mobility of cell-surface glycoconjugates during myogenesis the binding of fluorescein-conjugated plant lectins to myoblasts and myotubes of the L6 rat skeletal muscle cell line has been studied. Binding has been carried out at 4 degrees C on either live or glutaraldehyde-fixed cells. Fluorescein conjugates of soybean(More)
SIMCAP (Simulation-based Capacity Analysis Platform) is a simulation-based software tool designed to support analysis of intermodal terminal operation, specifically with respect to track capacity and yard capacity. The application was designed with a modular, extensible software architecture with an emphasis on capturing the primary complexities that exist(More)
Simulation modeling that has been successfully used to analyze intermodal capacity issues for a wide variety of facilities. Simulation technology provides an analysis mechanism for large intermodal facilities that are difficult to duplicate with other methods due to the interaction of many variables. Automation Associates, Inc. (AAI) is a simulation(More)
In the industrial engineering community, it's a well-known adage that focusing on <i>process</i> can help achieve better results. In this second of a series of papers, we'll focus on the <i>process of simulation testing</i> and outline how improving your testing process can lead to better results for your projects. We'll consider model building as a(More)
An intermodal rail terminal is a facility where the transfer of cargo occurs between truck and rail. The operations within these terminals involve many resources and operating rules. The ability of a terminal to respond to activity peaks that occur as a result of train arrivals and departures is critical. In order to explore how operations can be improved(More)